目的:建立同时测定双氯达克乳膏中氯霉素、丙酸氯倍他索和防腐剂羟苯乙酯含量的方法.方法:采用高效液相色谱法,以醋酸氟轻松为丙酸氯倍他索的内标.色谱柱为XBridgeTMC18,流动相为0.02 mol/L磷酸二氢钾-甲醇(35∶65),流速为1.0 ml/min,紫外检测波长为240nm,柱温为40℃.结果:氯霉素、丙酸氯倍他索和羟苯乙酯检测质量浓度线性范围分别为0.11～2.22、0.005～0.11、0.01～0.22 mg/ml(r均≥0.999 9,n=5),平均回收率分别为101.40％、98.31％、100.19％,RSD分别为0.70％、0.63％、0.75％(n=9).结论:该方法准确度和回收率均符合要求,可用于控制双氯达克乳膏的质量.%OBJECTIVE:To establish a method for simultaneous determination of chloramphenicol,clobetasol propionate and ethylparaben in Shuanglv dake cream.METHODS:HPLC method was adopted using fluocinonide as internal standard for clobetasol propionate.The determination was carried out on XBridgeTMC18 column with 0.02 mol/L potassium dihydrogen phosphate-methanol (35 ∶ 65) as mobile phase at flow rate of 1.0 ml/min.The UV detection wavelength was 240 nm,and the column temperature was 40 ℃.RESULTS:The linear ranges of chloramphenicol,clobetasol propionate and ethylparaben were 0.11-2.22 mg/ml,0.005-0.11 mg/ml and 0.01-0.22 mg/ml (r≥0.999 9,n=5),respectively.The average recoveries were 101.40％ for chloramphenicol (RSD=0.70％),98.31％ for clobetasol propionate (RSD=0.63％) and 100.19％ for ethylparaben (RSD=0.75％),respectively (n=9).CONCLUSIONS:The accuracy and recovery of the method are in line with the standard.It can be applied for the quality control of Shuanglv dake cream.
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