OBJECTIVE: To study the chemical structure of carrot pectin and to determine the content of it. METHODS: The average molecular weight was determinate by HPGPC; Paper chromatography (PC) and HPLC were used to identify the major monosaccharide component of pectin; UV and IR spectroscopy indentiry the chemical structure; the content of pectin was determined by sulfuric acid-cardyl method. RESULTS: The average molecular weight of carrot pectin was 1 750 625 and monosaccharide mainly composed of D-galactolipin and D-galacturonic acid; monosaccharide was mainly connected by β-pyran type glycosidic bonds. The content of pectin to galacturonic acid was 66.06%. CONCLUSION: The carrot pectin extract has large content of poly-saccharide and obvious chemical structure. The trial provides theory and trial basis for further development of carrot pectin.%目的:研究胡萝卜果胶的化学结构特征,并测定其含量.方法:采用高效凝胶渗透液相色谱法测定胡萝卜果胶的相对分子质量；纸色谱、高效液相色谱法鉴别果胶中主要单糖组成；紫外、红外光谱法鉴别其化学结构特征；硫酸-咔唑法测定果胶的含量.结果:胡萝卜果胶的平均相对分子质量为1750625,主要单糖组成为D-半乳糖和D-半乳糖醛酸；单糖的连接方式主要为β-吡喃型糖苷键连接.果胶含量以半乳糖醛酸计为66.06％.结论:胡萝卜果胶提取物多糖含量较高、化学结构特征明显.本试验结果可为胡萝卜果胶的进一步开发提供理论和试验基础.
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