Objective :The effects of endurance exercise on HIF‐1α/iNOS signaling in adipose tissue of high fat diet feeding mice were investigated to explore the molecular mechanisms of exercise‐induced prevention from adipose tissue inflammation induced by high fat diet .Meth‐ods :40 8‐week old C57BL/6 mice were randomly divided into 4 groups ,namely normal diet control (NC) ,normal diet exercise (NE) ,high fat diet control (HC) and high fat diet exercise (HE) with each group 10 mice .Exercise group were subjected to 8 week treadmill running program at a speed of 13 m/min .ELISA assay was used to measure serum TNF‐α and IL‐6 . RT‐PCR was used to determine gene expression while Western Blot was used to determine protein expression .Results :Compared to NC group ,serum TNF‐α ,IL‐6 and TNF‐α ,IL‐6 ,IL‐1β ,HIF‐α ,PDK1 ,GLUT1 ,LDHA ,iNOS mRNA expression as well as HIF‐1α and iNOS protein expression in adipose tissue SVCs of HC group were significantly elevated ( P<0 .05) . Campared with NC group ,HIF‐1α mRNA and protein expression in adipose tissue SVCs of NE group were reduced markedly (P< 0 .05) while other indicators changed without signifi‐cance .Compared with HC group ,serum TNF‐α ,IL‐6 and TNF‐α ,IL‐6 ,IL‐1β ,HIF‐α , PDK1 ,GLUT1 ,LDH‐A ,iNOS mRNA expression as well as HIF‐1α and iNOS protein ex‐pression in adipose tissue SVCs of HE group were significantly reduced ( P< 0 .05 ) .Conclu‐sion :8 week endurance treadmill running efficiently prevent chronic inflammation of high fat di‐et feeding mice possibly by inhibiting HIF‐1α/iNOS signaling pathway .%目的：研究耐力运动对高脂饮食小鼠脂肪组织 HIF‐1α／iNOS信号的影响，探讨运动预防脂肪组织炎症的分子机制。方法：8周龄C57BL／6小鼠40只随机分为正常饮食对照组（NC）、正常饮食运动组（NE）、高脂饮食对照组（HC）、高脂饮食运动组（HE），运动组进行8周跑台运动（13 m／min ）。 ELISA 法测定血清 TNF‐α、IL‐6，RT‐PCR 法测定基因表达， Western Blot法测定蛋白表达。结果：与 NC组相比，HC组小鼠血清 TNF‐α、IL‐6浓度显著升高（P＜0．05），脂肪组织 SVCs TNF‐α、IL‐6、IL‐1β、HIF‐1α、PDK1、LDH‐A、GLUT1、iNOS mRNA表达均显著升高（ P＜0．05），HIF‐1α和 iNOS 蛋白表达显著升高（ P＜0．05）；与 NC组相比，NE组小鼠SVCs HIF‐1α mRNA和蛋白表达显著下降（ P＜0．05），其余参数无显著差异；与 HC组相比，HE组血清 TNF‐α、IL‐6显著下降（P＜0．05），脂肪组织SVCs TNF‐α、IL‐6、IL‐1β、HIF‐1α、PDK1、LDH‐A、GLUT1、iNOS mRNA表达显著下降，HIF‐1α、iNOS蛋白表达显著下降（P＜0．05）。结论：8周耐力跑台运动可有效预防高脂饮食小鼠脂肪组织慢性炎症，其机制可能与运动抑制脂肪组织巨噬细胞 HIF‐1α／iNOS信号轴有关。
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