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METHOD FOR MEASURING DISTRIBUTION OF MACROPHAGE PATTERN INDUCIBLE NITRIC OXIDE SYNTHASE IN FATTY LIVER OF MOUSE WITH HIGH FAT DIET-INDUCED OBESITY
METHOD FOR MEASURING DISTRIBUTION OF MACROPHAGE PATTERN INDUCIBLE NITRIC OXIDE SYNTHASE IN FATTY LIVER OF MOUSE WITH HIGH FAT DIET-INDUCED OBESITY
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机译：高脂饮食引起的肥胖小鼠脂肪肝中巨噬细胞型可诱导型一氧化氮合酶分布的测定方法
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摘要
Disclosed is a method for measuring distribution of macrophage pattern inducible nitric oxide synthase in fatty liver of a mouse with high fat diet-induced obesity. The method for measuring distribution of macrophage pattern inducible nitric oxide synthase in fatty liver of the mouse with high fat diet-induced obesity comprises the steps of: preparing a three-week-old C57BL/6 mouse; feeding two types of high fat feeds (D-60 group: 60% calorie, D-45 group: 45% calorie) to the mouse for 15 weeks in a specific pathogen blocking facility; collecting liver tissue of the fed mouse; and measuring an amount of macrophage pattern inducible nitric oxide synthase from the collected liver tissue by applying an immunohistochemical dyeing method.;COPYRIGHT KIPO 2015
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