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首页> 中文期刊> 《体育科学》 >游泳运动对糖尿病小鼠骨骼肌AMPK/SIRT1／NF-κB炎症信号通路的影响

游泳运动对糖尿病小鼠骨骼肌AMPK/SIRT1／NF-κB炎症信号通路的影响

         
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摘要

目的：探讨耐力运动对糖尿病小鼠骨骼肌 AM PK／SIRT 1／NF‐κB炎症信号通路及相关炎症因子的影响，以其从骨骼肌慢性炎症的角度为肥胖、糖尿病等代谢类疾病的防治提供新的研究靶向。方法：采用4周高脂膳食喂养加注射链脲佐菌素（Streptozotocin ，STZ ）的方法构建糖尿病小鼠模型，建模成功后随机分为安静对照组（C ）、运动对照组（ E ）、糖尿病安静组（D ）、糖尿病运动组（DE ）。 E组和 DE组进行6周游泳耐力运动，1 h／天，5天／周。末次运动结束，空腹12 h后处死小鼠并采样。 RT‐PCR及 Western Blotting 技术检测相关基因的mRNA及蛋白表达水平。结果：1）与C组相比，D组小鼠体重极显著降低（ P＜0．01），空腹血糖显著升高（ P＜0．01）；与C组相比，E组小鼠体重显著降低（ P＜0．05）；与D组相比，DE组小鼠空腹血糖显著降低（P＜0．05）；与 E组相比，DE组小鼠体重极显著性降低（P＜0．01），空腹血糖极显著性升高（ P＜0．01）。2）与C组相比，D组小鼠IL‐10mRNA表达极显著性降低（ P＜0．01），E组IL‐6 mRNA 表达显著性升高（ P＜0．05），IL‐10 mRNA 表达极显著性升高（P＜0．01）；与D组相比，DE组 TNF‐α mRNA表达显著降低（P＜0．05），IL‐10 mRNA表达极显著性升高（ P＜0．01）；与 E组相比，DE组 IL‐6 mRNA 表达显著性下降（ P＜0．05）。3）与C组相比，D组 AMPK蛋白表达和 AMPK活性显著性下降（P＜0．05），NF‐κBp65 mR‐NA表达显著性升高（P＜0．05）；与D组相比，DE组 AMPKα2及 SIRT1 mRNA 表达极显著性升高（P＜0．01），AMPK 及 p‐AMPK蛋白表达和 AMPK 活性显著性升高（P＜0．05），NF‐κBp65蛋白表达极显著性下降（P＜0．01）；与 E组相比，DE组SIRT1 mRNA 表达极显著性升高（P＜0．01）。结论：长期耐力运动可抑制糖尿病小鼠骨骼肌中促炎因子的基因表达，促进抗炎因子的基因表达，同时，AM PK／SIRT 1／NF‐κB炎症信号通路的被抑制效应得到缓解。%Objective :The purpose of the present study was to investigate the effect of endur‐ance exercise on inflammatory factors and corresponding signaling pathways AMPK /SIRT1/NF‐κB in the skeletal muscles of diabetic mice ,hoping to provide new evidence for the preven‐tion and treatment of obesity and diabetes from the perspective of inflammation of the skeletal muscle .Methods :The diabetic mice were established by feeding with high‐fat diet for 4 weeks ,and then injected with Streptozotocin .All the mice were randomly chosen and assigned to either control group (C ,n= 8) or exercise group (E ,n= 8) ,diabetic group (D ,n= 8) or diabetic exercise group (DE ,n= 8) ,Groups E and DE were then interfered with 6‐week swim‐ming exercise of moderate intensity ,one hour per day ,five days per week .RT‐PCR was used to measure the mRNA expression of TNF‐α ,CCL2 ,IL‐6 ,IL‐10 ,AMPKα1 ,AMPKα2 ,SIRT1 and NF‐κBp65 .Western Blotting was used to measure the protein level of AMPK ,p‐AMPK , SIRT1 ,NF‐кBp65 and AC‐NF‐кBp65 .Results :1 ) Compared with group C ,the weight of mice in group D decreased significantly ( P< 0 .01 ) ,meanwhile the fasting blood glucose in‐creased significantly ( P< 0 .01 );Compared with group C ,the weight of mice in group E de‐creased significantly ( P< 0 .05 );Compared with group D ,the fasting blood glucose in group DE decreased significantly ( P< 0 .05 );Compared with group E ,the weight of mice in group DE decreased significantly ,while the fasting blood glucose increased significantly (P< 0 .01) . 2 ) Compared with group C ,the IL‐10 mRNA expression in group D was decreased significant‐ly(P< 0 .01);while ,the IL‐6 mRNA expression in group E was increased significantly (P<0 .05);Compared with group D ,the TNF‐αmRNA in group DE was decreased significantly (P < 0 .05 ) ,the IL‐10 mRNA in group DE was increased significantly ( P< 0 .01 );Compared with group E ,the IL‐6 mRNA of group DE was decreased significantly (P< 0 .05) .3) Com‐pared with group C ,the protein expression and activity of AMPK were decreased significantly (P< 0 .05) in group D ,while the mRNA of NF‐κBp65 was increased significantly (P< 0 .01) in group D ;Compared with group D ,the mRNA of AMPKα2 and SIRT1 were increased signif‐icantly ( P<0 .01 ) in group DE ,the protein expression of AM PK 、p‐AM PK and the activity of AMPK were increased significantly (P< 0 .05) ,while the protein expression of NF‐κBp65 was decreased significantly ( P< 0 .01 );Compared with group E ,the mRNA of SIRT 1 increased significantly ( P< 0 .01 ) .Conclusions :6 weeks regular swimming exercise can promote the mRNA expression of anti‐inflammatory factors ,while the pro‐inflammatory factors were re‐pressed in skeletal muscles of diabetic mice .What’s more ,exercise can reverse relieve the sup‐pression of AMPK/SIRT1/NF‐кB by diabetes .

著录项

  • 来源
    《体育科学》 |2016年第9期|40-47|共8页
  • 作者

    张坦; 崔迪; 张喆; 孙易; 丁树哲;

  • 作者单位

    华东师范大学 青少年健康评价与运动干预教育部重点实验室;

    上海 200241;

    华东师范大学 体育与健康学院;

    上海 200241;

    华东师范大学 青少年健康评价与运动干预教育部重点实验室;

    上海 200241;

    华东师范大学 体育与健康学院;

    上海 200241;

    华东师范大学 青少年健康评价与运动干预教育部重点实验室;

    上海 200241;

    华东师范大学 体育与健康学院;

    上海 200241;

    华东师范大学 青少年健康评价与运动干预教育部重点实验室;

    上海 200241;

    华东师范大学 体育与健康学院;

    上海 200241;

    华东师范大学 青少年健康评价与运动干预教育部重点实验室;

    上海 200241;

    华东师范大学 体育与健康学院;

    上海 200241;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 运动生物化学;
  • 关键词

    耐力运动; 糖尿病; 骨骼肌; 炎症; AMPK/SIRT1/NF-кB;

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