In order to clarify the effects of Buchnera aphidicola on growth and development of M.persicae in different vegetables,we took vegetables of 3 different families (radish of Raphanus sativus L.,pepper of Capsicum annuum L.,and cucumber of Cucumis sativus L.) as experimental material,and compared the growth and reproduction situation of M.persicae untreated and treated with Rifampicin.The results showed that the DNAK gene expression quantity of Buchnera in aphids treated with different concentrations of rifampiein was significantly decreased.After treated with 200 μ g · mE-1 rifampicin,the reproduction quantity,life span and body weight of aphids were significantly lower than that of the healthy aphids,indicating that endosymbiont Buchnera played a very important role in the growth and development of M.persicae,and the reduction of endosymbiont could have negative effect on M.persicae.%为明确桃蚜内共生菌Buchnera aphidicola对桃蚜生长发育和繁殖的影响,为有效控制桃蚜提供新的思路,本试验以十字花科(萝卜)、茄科(辣椒)和葫芦科(黄瓜)蔬菜为试材,比较健康桃蚜和利福平处理后的桃蚜在3种蔬菜上的生长发育和繁殖情况.结果表明,不同浓度利福平处理后桃蚜Buchnera的DNAK基因表达量均显著降低;200 μg·mL-1利福平处理后的桃蚜产蚜量、寿命和体重都显著低于健康桃蚜.说明桃蚜的内共生菌Buchnera在其生长发育过程中起着非常重要的作用,降低内共生菌的量对桃蚜可产生不利影响.
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