采用高效液相色谱法,对心里美、超级郑研、沙窝萝卜3个水果萝卜品种肉质根和叶片的硫代葡萄糖苷组分与含量进行了鉴定和分析.结果表明,3个水果萝卜品种肉质根和叶片中均检测到9种硫苷组分,其中包括6种脂肪族硫苷(4-甲基亚磺酰-3-丁烯基硫苷、2-丙烯基硫苷、4-甲基亚磺酰丁基硫苷、5-甲基亚磺酰戊基硫苷、4-甲硫基丁基硫苷、4-甲硫基-3-丁烯基硫苷)和3种吲哚族硫苷(4-甲氧基吲哚甲基硫苷、吲哚-3-甲基硫苷、1-甲氧基吲哚甲基硫苷),但不同品种及器官之间的硫苷含量存在明显差异.3个水果萝卜品种肉质根总硫苷含量分别为22472.84、13585.86、28200.70μg·g-1(DM),其中95%以上是脂肪族硫苷;肉质根总硫苷含量分别是叶片的4.56、2.71、4.55倍;肉质根和叶片中主要脂肪族硫苷组分均为4-甲基亚磺酰-3-丁烯基硫苷,分别占总硫苷含量的90.11%~93.92%和63.03~73.72%.%The composition and content of glucosinolates(referred to as'GSL')in leaf and root of 3 fruity radishes'Xinlimei','super Zhengyan','Shawo'were identified and analyzed by HPLC method. The results showed that 9 glucosinolates compositions in fleshy roots and leaf blades were detected,including 6 kinds of aliphatic glucosinolates(4-methyl sulfonyl-3-butylene glucosinolate,2-allyl glucosinolate,4-methyl sulfonyl butyl glucosinolate,5-methyl sulfonyl amyl glucosinolate,4-methyl-butyl glucosinolate,4-methyl thio-3-butylene glucosinolate)and 3 kinds of indole glucosinolates(4-methoxy indole methyl glucosinolate, indolymethyl-3-butenyl glucosinolate,1-methoxy methyl indole glucosinolate).But,there existed significant differences in total sulfur glycoside contents between different varieties and organs.The total glucosinolates contents in fleshy roots of the 3 fruity radishes were 22472.84,13585.86,28200.70 μg·g-1(DM), among which over 95% was total aliphatic sulfid.The total glucosinolates contents in fleshy root of the fruity radish were 4.56,2.71,4.55 times of that in leaf blades,respectively.The main aliphatic sulfide component in fleshy root and leaf blade was 4-methyl thio-3-butylene glucosinolate,accounting for 90.11%~93.92% and 63.03%~73.72% in the total glucosinolates content,respectively.
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