Objective To investigate the expression of C17orf76-AS1 between normal ovarian epithelial cell and epithelial ovarian cancer cells and the effect of C17orf76-AS1 overexpression on the biological function of SKOV3 cells.Methods The quantitative real-time PCR(QPCR) was used to detect the expression of C17orf76-AS1 in normal ovarian cell (IOSE80) and ovarian cancer cells (A2780,SKOV3,OVCAR3,HO-8910 and HO-8910PM).Construction of C17orf76-AS1 overexpression plasmid based on pcDNA3.1 plasmid and transient transfection of ovarian cancer cell SKOV3 with Lipo2000 were carried out.QPCR was used to detect the overexpression efficiency of C17orf76-AS1 in SKOV3 cells.CCK-8 was used to detect the proliferation of SKOV3.Transwell and wound healing assay were used to detect the migration of SKOV3.Results Compared with IOSE80, C17orf76-AS1 showed low expression in A2780,SKOV3,OVCAR3,HO-8910 and HO-8910PM about 0.36,0.30,0.46,0.32 and 0.31 folds(P<0.05).The proliferation of SKOV3 cells significant decreased about 1.5 folds after C17orf76-AS1 overexpression with CCK-8 assay.The Transwell assay demonstrated C17orf76-AS1 overexpression significant decreased the migration of SKOV3 by about 1.3 times, and the wound healing assay confirmed that the healing ability of SKOV3 was significantly lower than that of the control group after the overexpression of C17orf76-AS1(P<0.05).Conclusion LncRNA C17orf76-AS1 was downregulated in ovarian cancer cells.The overexpression of C17orf76-AS1 significant decreased the proliferation and migration of ovarian cancer cell.%目的 检测C17orf76-AS1在正常卵巢上皮细胞与卵巢上皮癌细胞系中的表达情况并探讨C17orf76-AS1过表达对卵巢上皮癌细胞系SKOV3生物学功能的影响.方法 通过定量PCR(QPCR)方法检测C17orf76-AS1在在卵巢上皮细胞IOSE80及不同卵巢上皮癌细胞系(A2780、SKOV3、OVCAR3、HO-8910和HO-8910PM)中的表达.在pcDNA3.1基础上构建C17orf76-AS1过表达质粒并采用Lipo2000瞬时转染SKOV3细胞,QPCR检测SKOV3细胞中C17orf76-AS1的过表达效率;CCK-8实验检测C17orf76-AS1过表达对SKOV3细胞增殖的影响;Transwell法和划痕实验检测C17orf76-AS1过表达对SKOV3细胞侵袭迁移能力的影响.结果 与正常卵巢上皮细胞IOSE80比较,C17orf76-AS1在A2780、SKOV3、 OVCAR3、HO-8910及HO-8910PM细胞中均呈低表达,分别下降了0.36、0.30、0.46、0.32、0.31倍(P<0.05).在SKOV3细胞中过表达C17orf76-AS1,CCK-8结果显示C17orf76-AS1过表达48 h后,SKOV3的增殖能力显著下降了约1.5倍;Transwell结果显示SKOV3细胞迁移能力下降约1.3倍,划痕实验也同样证实过表达C17orf76-AS1后,SKOV3细胞愈合能力显著低于对照组(P<0.05).结论 C17orf76-AS1在卵巢上皮癌细胞中异常低表达,其过表达显著降低了卵巢癌细胞增殖和迁移能力.
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