Objective To explore the expression and clinical significance of E-cadherin and β-catenin in osteosarcoma tissues.Methods From April 2012 to October 2015,54 specimens of osteosarcoma tissues and 22 specimens of osteochondroma tissues were enrolled.Immunohistochemistry was used to detect the expression of E-cadherin and β-catenin in those above tissues.The correlation of the expression of E-cadherin,β-catenin and clinical parameters of osteosarcoma were analyzed.Results In 54 specimens of osteosarcoma tissues,the positive rate of E-cadherin protein was 35.2％ (19/54),significantly lower than 68.2％ (15/ 22) in osteochondroma tissues (P＜0.05).However the positive rate of β-catenin protein was 68.5％ (37/54),significantly higher than 9.1％ (2/22) in osteochondroma tissues (P＜0.05).The expression of E-cadherin protein was associated with Enneking stage and metastasis (P＜0.05).The expression of β-catenin protein was associated with tumor size,Enneking stage and metastasis (P＜0.05).Moreover,the expression of E-cadherin and β-catenin was negatively correlated (r=-0.764,P＜0.05).Conclusion E-cadherin and β-catenin are abnormally expressed in osteosarcoma tissues,and they are correlated to Enneking stage and metastasis.Both of them play a role in the development and progression of osteosarcoma.%目的 探讨上皮型钙粘蛋白(E-cadherin)和β-连环蛋白(β-catenin)在骨肉瘤组织中的表达及其临床意义.方法 收集2012年4月至2015年10月54例骨肉瘤和22例骨软骨瘤组织标本.采用免疫组织化学法检测上述组织中E-cadherin和β-catenin的表达,并分析两者的表达与骨肉瘤临床病理特征的关系.结果 骨肉瘤组织中E-cadherin蛋白的阳性表达率为35.2％(19/54),显著低于骨软骨瘤组织的68.2％(15/22),差异有统计学意义(P＜0.05).骨肉瘤组织中β-catenin蛋白的阳性表达率为68.5％(37/54),显著高于骨软骨瘤组织的9.1％(2/22),差异有统计学意义(P＜0.05).E-cadherin蛋白的表达与Enneking分期和转移有关(P＜0.05);β-catenin蛋白的表达与肿瘤大小、Enneking分期及转移有关(P＜0.05).此外,E-cadherin与β-catenin蛋白在骨肉瘤组织中的表达呈负相关(r=-0.764,P＜0.05).结论 E-cadherin和β-catenin在骨肉瘤组织中异常表达,且均与Enneking分期和转移有关,可能与骨肉瘤的发生、发展有关.
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