Objective To investigate and analyze the expression of miR-21 in hepatocarcinoma cell strains and the relation-ship with PTEN. Methods The quantitative reverse transcription-PCR were used to detect the expression of miR-122 and PTEN in hepatocarcinoma cell strains, PTEN and p-Akt expression was detected in tumor-bearing nude mice treated with miR-21 inhibitor. Re-sults The relative expression of miR-21 was 2�97±0�79,137�66±23�60 and 11�21±1�14 in WRL-68, MHCC97-H and SMMC-7721strains respectively, while that was 1�29 ± 0�33, 36�90 ± 11�84 and 6�22 ± 0�85 respectively when miR-21 inhibitor was transfected. The relative expression of PTEN was 2�88 ± 0�93, 0�19 ± 0�07 and 0�62 ± 0�06 in WRL-68, MHCC97-H and SMMC-7721strains respectively, while that was 6�11±1�07, 2�65±0�66 and 4�32±0�84 respectively when miR-21 inhibitor was transfected. The expression of PTEN was positive and p-Akt was down regulated when tumor-bearing nude mice was treated with miR-21 inhibitor. Conclusion MiR-21 is an upstream regulation factor of PTEN, it can affect PTEN expression.%目的:探讨miR-21在肝癌细胞中的表达水平及其与PETN的关系。方法采用荧光定量PCR分别检测人肝细胞株WRL-68和肝癌细胞株MHCC97-H、SMMC-7721中miR-21和PTEN的表达;建立人肝癌裸鼠皮下移植瘤模型,给予miR-21抑制剂后,检测移植瘤中PTEN蛋白表达和下游信号p-Akt蛋白表达。结果人肝细胞株WRL-68和肝癌细胞株MH-CC97-H、SMMC-7721中miR-21的相对表达量(以U6为内参基因)分别为2�97±0�79、137�66±23�60、11�21±1�14,PTEN相对表达量分别为2�88±0�93、0�19±0�07、0�62±0�06;3种细胞转染miR-21抑制剂后,miR-21的相对表达量分别为1�29±0�33、36�90±11�84、6�22±0�85,PTEN相对表达量分别为6�11±1�07、2�65±0�66、4�32±0�84。人肝癌裸鼠皮下移植瘤模型给予miR-21抑制剂后,MHHC97-H细胞中PTEN表达阳性,p-Akt表达下调。结论 miR-21是PTEN的上游调控因子,调节miR-21能引起PTEN的改变,继而调控Akt信号途径和细胞的增殖活性。
展开▼