目的 分析Ki-67、EGFR、HER-2、p53在乳腺癌组织中的表达,并探讨其与乳腺癌临床病理特征之间的相关性.方法 用免疫组化法检测138例乳腺癌患者病理组织中Ki-67、EGFR、HER-2和p53蛋白的表达,并结合临床病理特征进行相关性分析.结果 Ki-67、EGFR、HER-2和p53在乳腺癌组织中的表达率依次为91.30%、17.39%、62.32%和23.19%,不同年龄组其表达均无统计学差异(P＞0.05).Ki-67的表达与组织学分级、病理类型、肿块大小、淋巴结是否转移及分期显著相关(P＜0.05);EGFR表达与病理类型和ER状态显著相关(P＜0.05);HER-2表达与病理类型、组织学分级、淋巴结转移、远处转移及分期无相关性(P＞0.05);而p53表达与组织学分级、ER和PR状态呈显著相关(P＜0.05).ER和PR呈正相关;EGFR、HER-2、p53与ER均为负相关;HER-2和PR呈负相关;Ki-67与EGFR、HER-2、p53、ER及PR之间均无相关性;p53与EGFR呈正相关;EGFR与HER-2呈负相关.结论 联合检测乳腺癌组织中EGFR、Ki-67、HER-2 和p53蛋白的表达能更清楚地了解乳腺癌的生物学行为,对乳腺癌的诊断、指导治疗及评价预后有重要的临床意义.%Objective To investigate the expression of EGFR, Ki-67, HER-2 and p53 in breast cancer and their correlation with clinicopathological factors.Methods Immunohistochemistry was used to detect the expression of EGFR, Ki-67, HER-2 and p53 in 138 cases of breast cancer tissues and the correlation with pathological factors were analyzed.Results The positive expression of EGFR, Ki-67, HER-2 and p53 was 91.30％, 17.39％ ,62.32％ and 23.19％, respectively.The expressions of EGFR, Ki-67, HER-2 and p53 were not statistically correlated with age (P ＞ 0.05 ).Ki-67 expression was statistically correlated with grade, pathology, tumor size, lymph node metastasis and stage( P ＜ 0.05 ); EGFR expression was significantly correlated with pathology and ER ( P ＜ 0.05 );HER-2 expression was not significantly correlated with pathology, grade, lymph node metastasis and stage( P ＞0.05) ;p53 expression was significantly correlated with grade, ER and PR( P ＜ 0.05 ).ER positively correlated with PR; EGFR, HER-2 and p53 negatively correlated with ER.HER-2 negatively correlated with PR.No significant correlation was found among Ki-67 and EGFR, HER-2, p53,ER, PR.p53 positively correlated with EGFR, while EGFR negatively correlated with HER-2.Conclusion The detection of EGFR,Ki-67, HER-2 and p53 protein expression in breast cancer is helpful to a better understanding of the biological behavior of breast cancer with diagnosis, treatment and prognostic evaluation.
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