Objective To investigate the level of microRNA-155-5p (miR-155-5p) in hepatocellular carcinoma (HCC) tissues and its relationship with clinicopathological parameters and prognosis.Methods A total of 114 pairs of HCC tissues and paracancerous tissues were collected from January 2013 to December 2015.The levels of miR-155-5p in the above tissues were detected by real-time fluorescence quantitative PCR (QPCR). The relationships between miR-155-5p levels and the clinicopathological parameters of HCC (sex, age, number of tumor, tumor size, degree of differentiation, tumor capsule, TNM stage, Child-Pugh grade and alpha-fetoprotein level) were analyzed.According to the follow-up data, the relationship between different levels of miR-155-5p and prognosis of HCC patients was analyzed.Results The results of QPCR showed that the level of miR-155-5p in HCC tissues was 3.041±1.393, which was higher than 1.167±0.514 in paracancerous tissues (P＜0.05). The level of miR-155-5p was correlated with the number of tumor, tumor size, degree of differentiation and TNM stage in HCC patients (P＜0.05), but not with sex, age, tumor capsule, ChildPugh grade and alpha-fetoprotein level (P＞0.05). HCC patients were followed up for 8 to 60 months, with a median follow-up time of36.0 months and a median overall survival (OS) of 46.0 months.The median OS of 60 cases with high-expression of miR-155-5p was37.0 months, and that of 54 cases with low-expression was 57.0 months.The median OS of low-expression of miR-155-5p was higher than that of high-expression (P＜0.05). Conclusion The up-regulation of miR-155-5p in HCC tissues is involved in the occurrence and development of HCC.The level of miR-155-5p with high tumor load, poor differentiation and late staging is higher, and the prognosis of high miR-155-5p level is worse.This molecule may be a potential prognostic indicator of HCC.%目的 探讨肝细胞癌(HCC)组织微小RNA-155(miR-155-5p)的水平及其与临床病理特征和预后的关系.方法 收集本院2013年1月至2015年12月经手术切除的114对HCC组织和癌旁组织,采用荧光实时定量PCR(QPCR)检测以上组织的miR-155-5p水平,分析miR-155-5p水平与HCC临床病理特征(性别、年龄、肿瘤数目、肿瘤大小、分化程度、肿瘤包膜、TNM分期、Child-Pugh分级和甲胎蛋白水平)的关系,根据随访资料分析不同miR-155-5p水平与HCC患者预后的关系.结果 QPCR检测结果 显示HCC组织的miR-155-5p水平为3.041±1.393,高于癌旁组织的1.167±0.514(P＜0.05).miR-155-5p水平与HCC患者的肿瘤数目、肿瘤大小、分化程度和TNM分期有关(P＜0.05),而与性别、年龄、肿瘤包膜、Child-Pugh分级和甲胎蛋白水平均无关(P＞0.05).HCC患者全组获随访8～60个月,中位随访时间36.0个月,中位总生存期(OS)为46.0个月,其中miR-155-5p高表达60例的中位OS为37.0个月,低表达54例的中位OS为57.0个月,miR-155-5p低表达的中位OS大于高表达者(P＜0.05).结论 HCC组织miR-155-5p水平上调参与HCC的发生发展,肿瘤负荷大、分化较差及分期较晚的miR-155-5p水平较高,而高miR-155-5p水平的预后较差,该分子可能作为一个评估HCC预后的潜在指标.
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