目的 研究辽西地区汉族人群中单核细胞趋化蛋白-1(MCP-1)-2518A/G基因多态性与急性心肌梗死(AMI)的相关性.方法 收集2010年9月-2011年6月于辽宁医学院附属第一医院心内科住院的无血缘关系的150例AMI患者(病例组)和同期159例健康体检者(对照组)全血.采用聚合酶链反应限制性片段长度多态性(PCR-RFLP)技术检测MCP-1的基因型;ELISA法检测AMI患者和健康对照者血清MCP-1的水平.结果 AA、AG、GG 3种基因型在病例组和对照组间分布差异有统计学意义(P＜0.01).等位基因A、G的频率在病例组和对照组间分布差异有统计学意义(P＜0.01);血清MCP-1水平在病例组与对照组间、病例组内不同基因型间的分布差异均有统计学意义(P＜0.01).结论 MCP-1-2518A/G基因多态性与AMI的发病具有相关性,G等位基因可能是辽西地区人群AMI的遗传易感基因,但这种联系可能和传统危险因素协同存在.%Objective To investigate the correlation of MCP - 1 - 2518 A/G gene polymorphism with acute myocardi-al infarction ( AMI) in Chinese Han population in Liaoxi Province. Methods Totally 150 unrelated AMI inpatients in our department from September 2010 to June 2011 were selected as case group. Meanwhile, 159 healthy persons ( confirmed by physical check - ups ) during the same period were recruited as the controls. MCP - 1 genotype was detected by polymerase chain reaction - restriction fragment length polymorphism ( PCR - RFLP ). The serum level of MCP - 1 was determined by enzyme linked immunosorbent assay ( ELISA ). Results There was a significant difference in genotype distribution of three types of genes ( AA, AG, and GG ) between these two groups ( P <0. 01 ). The frequency distribution of A allele was significantly different from G allele ( P <0. 01 ). The distribution differences of serum level of MCP - 1 between the two groups, and between different genotypes in the case group were all significant ( P <0. 01 ). Conclusion The MCP - 1 -2518A/G gene polymorphism is associated with the pathogenesis of AMI, and G allele may be a genetic risk factor for AMI, although such relation may co - exist with the conventional risk factors.
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