Discovery and identification of quality markers of Sparganii Rhizoma based on zebrafish thrombosis model

Nan Xu; Rong Sun; Yin-ping Shi; Li-wen Han; Hai-yan Shi

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Discovery and identification of quality markers of Sparganii Rhizoma based on zebrafish thrombosis model

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Objective:The aim of the present study was to determine the quality marker(Q-Markers)of Sparganii Rhizoma against thrombus through an integration of investigations on its antithrombotic effect,content determination and spectrum-effect correlation analysis.Methods:Based on the concept of Q-Marker,Sparganii Rhizoma was investigated for the identification of chemical component.The pharmacological effects on arachidonic acid-induced thrombosis in zebrafish were also investigated.The material basis in ethanol extract was determined by HPLC-UV.Furthermore,the potential Q-Markers were analyzed and predicted according to the effect-chemical correlation analysis.Finally,the anti-thrombotic Q-Markers were verified through the anti-thrombotic test of monomer components.Results:The model of thrombosis zebrafish was established with larvae exposed to 100 mmol/L arachidonic acid for 1 h.Nine ingredients in Sparganii Rhizoma were identified as 5-hydroxymethylfurfural,vanillic acid,ferulic acid,p-hydroxybenzaldehyde,p-hydroxybenzoic acid,vanillin,protocatechuic acid,p-coumaric acid and isoferulic acid.According to the determination effect of zebrafish thrombosis model and HPLC content analysis results,all the other contents present positive correlation except 5-hydroxymethylfurfural,and the P values of three representative potential Q-Markers(ferulic acid,protocatechuic acid and p-coumaric acid)were 0.002,0.001 and 0.026,respectively.Conclusion:Sparganii Rhizoma showed a dose-dependent effect on the recovery of reducing cardiac red blood cell on zebrafish model.Three phenolic acids(ferulic acid,protocatechuic acid and p-coumaric acid)were proved to possess the anti-thrombotic effects which could be regarded as the potential QMarkers for quality assessment of Sparganii Rhizoma.

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《中草药：英文版》 |2021年第3期|389-395|共7页
作者
Nan Xu; Rong Sun; Yin-ping Shi; Li-wen Han; Hai-yan Shi;
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作者单位

Laboratory of Chinese Medicine Preparation;

Shandong Research Academy of Traditional Chinese Medicine;

Jinan 250014;

China;

Institute of Advanced Medical Sciences;

Shandong University;

Jinan 250014;

China;

Department of Clinical Pharmacy;

The First Affiliated Hospital of Shandong First Medical University;

Jinan 250014;

China;

Institute of Biology of Qilu University of Technology(Shandong Academy of Sciences);

Jinan 250014;

China;

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原文格式 PDF
正文语种 chi
中图分类中药化学;
关键词
anti-thrombotic activity; Q-Marker; Sparganii Rhizoma; zebrafish;

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Discovery and identification of quality markers of Sparganii Rhizoma based on zebrafish thrombosis model

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