通过Mn掺杂ZnS量子点表面的羧基与人IgG表面的氨基进行酰胺缩合,制备了人IgG功能化的Mn掺杂ZnS量子点( IgG-QDs)。通过金纳米粒子和羊抗人IgG之间的静电相互作用,合成了羊抗人IgG功能化的金纳米粒子。利用IgG-QDs和羊抗人IgG功能化的金纳米粒子之间的荧光共振能量转移效应,建立了人血清中人IgG的室温磷光检测新方法。本方法的线性范围为1~10μg/mL,检出限为0.45μg/mL。由于引入了室温磷光检测,本方法有效避免了机体自发荧光和基质散射光等背景信号的干扰,成功实现了人血清样品中人IgG的检测。%IgG functionalized Mn doped ZnS quantum dots ( IgG-QDs ) were fabricated via the amide condensation between the carboxy groups on the surface of Mn doped ZnS quantum dots and the amino groups on the surface of IgG. The IgG antibody functionalized Au nanoparticals were synthesized via the electrostatic interaction between the Au nanoparticals and IgG antibody. Based on the fluorescence resonance energy transfer effect between the IgG-QDs and the IgG antibody functionalized Au nanoparticals, the IgG-QDs could be applied for low background phosphorescent sensing of IgG with the linear range of 1-10 μg/mL and the limit of detection of 0. 45 μg/mL. The proposed method was successfully applied to detect IgG in human serum without the disturbance of the background fluorescence and scattered light.
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