High quality PEI-capped Mn-doped ZnS QDs were prepared via a microwave and ultrasonic-assisted synthesis for room temperature phosphorescence detection of guanosine 5 ' -triphosphate ( GTP). Such QDs showed high selectivity and sensitivity for GTP, and could distinguish GTP from adenosine triphosphate ( ATP ) , cytidine triphosphate ( CTP ) , uridine triphosphate ( UTP) , adeno-sine diphosphate ( ADP) and adenosine monophosphate ( AMP ) . The room temperature phosphorescence response of the PEI-capped Mn-doped ZnS QDs for GTP reached equilibrium in 3 min. PEI-capped Mn-doped ZnS QDs gave a detection limit(3s) of 0. 6 (xmol/L for GTP. The developed phosphorescence probe favors biological applications since the interference from scattering light and au-tofluorescence can be effectively eliminated. The developed method has been successfully applied for the room temperature phosphorescence detection of GTP in cancer cell extract.%利用微波-超声波辅助方法合成了水溶性聚乙烯亚胺包覆Mn掺杂ZnS量子点,并建立了基于该量子点的室温磷光检测三磷酸鸟苷(GTP)的新方法.该量子点对GTP具有较高的灵敏度和选择性,能区分GTP与其结构类似物三磷酸腺苷(ATP)、三磷酸胞苷(CTP)、三磷酸尿苷(UTP)、二磷酸腺苷(ADP)和一磷酸腺苷(AMP).该量子点的室温磷光对GTP的响应在3 min内达到平衡,其磷光增强值与GTP浓度呈良好的线性关系,对GTP的检出限为0.6μmol/L.该方法成功应用于癌细胞提取液中GTP的检测,为GTP的检测提供了新方法.
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