A silicon dioxide (SiO2) cavities array was fabricated on indium-tin oxide (ITO) electrode surface with the template of the polystyrene (PS) particles array by using sol-gel technique. The morphology of SiO2 cavities array was highly ordered which was obtained by scanning electrode microscope. The results of electrochemistrical study showed that the cyclic voltammetric (CV) curve of SiO2 cavities array was in accordance with that of microelectrodes array. Using hemoglobin (Hb) as model protein, an amperometric biosensor for detection of H2O2 was prepared by adsorbing Hb in SiO2 cavities directly. The properties of direct electrochemistry and electrocatalysis of Hb were studied by CV method. The response of the biosensor for H2O2 was fast. A linear relationship between current response and the concentration of H2O2 ranging from 2. 03 × 10~6 to 1. 21 × 10-2 mol/L was obtained with a detection limit of 5. 73×10-7mol/L. And the apparent Michaelis-Menten constant was 0. 266 mmol/L.%以聚苯乙烯(PS)微球阵列为模板,采用溶胶-凝胶法在氧化铟锡( ITO)电极上制备了二氧化硅(SiO2)球腔阵列,扫描电镜显示此方法制备的SiO2球腔阵列高度有序.电化学研究结果表明,该球腔阵列的循环伏安曲线符合微电极阵列的电化学特点.将血红蛋白(Hb)作为氧化还原模型蛋白直接吸附于球腔内,制得电流型过氧化氢(H2O2)生物传感器,研究了Hb在该微电极阵列上的直接电化学和电催化性质.所构建的传感器对H2 O2的响应快速灵敏,其线性范围为2.03×10-6～1.21×10-5mol/L和2.03×10-5～1.21×10-2mol/L;检出限为5.73× 10-7mol/L,米氏常数为0.266 mmol/L.
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