Objective To study the effect of human 8-hydroxyguanine deoxyribonucleic acid glycosidase (hOGG1) SNP rs1052133 and sperm DNA integrity on primary male infertility. Methods Based on case-control and case-only study design, polymerase chain reaction-restriction fragment length polymorphisms (PCR-RFLP) was used to measure the genotypes of rs1052133 among 332 primary male infertility patients and 329 controls. Sperm chromatin depression (SCD) test was used for the examination of sperm DNA integrity. The correlation between sperm DFI and sperm parameters as well as the effect of different rs1052133 genotypes to the genetic susceptibility of male infertility and DFI were analyzed. Results The DFI of primary male infertility was higher than that of fertile man, and DFI negatively correlated with the ratio of forward motile sperm and the ration of non-forward motile sperm(r = -0.35 and -0.13, P<0.05), but positively correlated with immotile sperm ( r =0.24, P<0.05) . The risk of rs1052133 CC genotype carriers were 1.93 fold higher than that of rs1052133 GG genotype. What’s more, there were no interaction effect between male infertility and cigarette smoking and alcohol consumption as well as no significant difference in sperm DFI among these three genotypes. Conclusion The sperm DNA integrity in male infertility patients were lower than that of fertile men. Sperm DNA damage which significant negative correlated with sperm quality was a cause of primary male infertility. Although the CC genotype of hOGG1 rs1052133 was associated with an increased risk of male infertility, it may be not associated with sperm DNA integrity.%目的：探讨人类8-羟基鸟嘌呤DNA糖苷酶（human 8-hydroxyguanine deoxyribonucleic acid glycosidase, hOGG1）基因rs1052133（Cys326Ser）及精子DNA完整性对原发性男性不育的影响。方法收集2011年8月至2012年12月间在宁夏医科大学总医院就诊的332例原发性男性不育患者和329例健康体检者分别作为病例组和对照组，采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测两组人群hOGG1 rs1052133基因型；采用精子染色质扩散实验（SCD）检测病例组和对照组精子DNA损伤程度。分析精子DNA完整性与精液参数的相关性以及hOGG1 rs1052133不同基因型与原发性男性不育的相关性以及对精子DNA完整性的影响。结果原发性男性不育患者精子DNA碎片指数（DFI）高于对照组；原发性男性不育患者精子DFI与前向运动精子和非前向运动精子呈负相关（r =-0.35和-0.13，P＜0.05），与不动精子呈正相关（r =0.24，P＜0.05）；携带hOGG1 rs1052133CC基因型的个体患原发性少弱精子症的风险是携带GG型个体的1.93倍。rs1052133与吸烟和饮酒与原发性男性不育的发病风险无交互作用，同时该位点三种不同基因型的患者间精子DFI差异无统计学意义。结论原发性男性不育患者精子DNA完整性降低，并且精子DNA损伤可导致精液质量下降，是原发性男性不育的病因之一。 hOGG1 rs1052133CC基因型可能是原发性男性不育的危险因素，但该位点可能不影响精子DNA完整性。
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