本试验旨在研究脱氧雪腐镰刀菌烯醇(DON)与玉米赤霉烯酮与(ZEA)联合暴露对体外培养鸡脾脏淋巴细胞内环境稳态的影响.分别以0.01250μg/mL DON+0.00625μg/mL ZEA、0.050μg/mL DON+0.025μg/mL ZEA、0.2μg/mL DON+0.1μg/mL ZEA、0.8μg/mL DON+0.4μg/mL ZEA对体外培养鸡脾脏淋巴细胞进行联合暴露培养,48 h后测定细胞膜ATP酶(Ca2+-ATP酶、Na+/K+-ATP酶)活性以及细胞内pH、Ca2+水平和钙调蛋白(CaM)的mRNA表达水平.同时设不添加毒素的空白对照组.结果表明:添加毒素的各试验组间,细胞内Ca2+水平、CaM mRNA表达水平随毒素浓度的升高而增加,且添加毒素的各试验组均显著或极显著高于空白对照组(P<0.05或P<0.01).细胞内pH以及细胞膜Ca2+-ATP酶与Na+/K+-ATP酶活性均随毒素浓度的升高而降低,且添加毒素的各试验组均显著或极显著低于空白对照组(P<0.05或P<0.01).由此得出,DON、ZEA联合暴露导致体外培养鸡脾脏淋巴细胞内酸化、离子平衡失调等一系列细胞内环境稳态失衡,且呈剂量依赖性.%The aim of this experiment was conducted to study the effects of combined exposure to deoxyni-valenol ( DON) and zearalenone ( ZEA) on the homeostasis of chicken splenic lymphocytes cultured in vitro. The chicken splenic lymphocytes were cultured in vitro with different doses of DON and ZEA in cultured fluid, and the combined exposure doses were DON 0. 01250 μg/mL and ZEA 0. 00625 μg/mL, DON 0.050 μg/mL and ZEA 0.025 μg/mL, DON 0.2 μg/mL and ZEA 0.1 μg/mL, DON 0.8 μg/mL and ZEA 0.4 μg/mL, individually. After cultured 48 hours, the activities of cellular membrane ATPases ( Ca2+-ATPase and Na+/K+-ATPase) , and the intracellular Ca2+level, pH and calmodulin ( CaM) mRNA expression level in chicken splenic lymphocytes were determined. And the blank contrast group was set separately. The results showed as follows: the intracellular Ca2+ level and CaM mRNA expression level in the treated groups were in-creased with the increase of toxin concentration, which were significantly or extremely significantly higher than those in the blank control group ( P<0.05 or P<0.01) . The intracellular pH and the activities of cellular mem-brane Ca2+-ATPase and Na+/K+-ATPase in the treated groups were decreased with the increase of toxin con-centration, which were significantly or extremely significantly lower than those in the control group ( P<0.05 or P<0.01) . In conclusion, combined exposure to DON and ZEA can lead to a series of intracellular homeo-stasis, such as intracellular acidification and imbalance of ion homeostasis, which is dose dependent.
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