This experiment was conducted to investigate the distribution and the dominant serotype's mutation of avian Salmonella in Eastern China. Using the methods of enrichment, purification, Polymerase Chain Reaction (PCR) , serotype identification and biochemical identification, 1 730 samples were detected and seven isolates with mutated serotypes were sequenced to analyze serotypes of avian Salmonella. The results showed that 87 isolates were identified as Salmonella and belong to five subtypes, including S. typhimurium, S. derby, S. saintpaul, S. pullorum and S. abaetetuba. Furthermore, Hd and Hg were mutated in seven S. pullorum isolates. These results indicated that poultry were mainly infected by S. pullorum in Eastern China. The serotype of the seven S. pullorum isolates mutated and the mutated rate was increasing. The research provided the experimental evidence of serotypes' distribution and mutated serotypes in Salmonella isolates.%本文旨在研究中国华东地区沙门菌分布情况及其优势菌的血清型变异情况.收集2010年10月-2012年8月来源于江苏、安徽、上海等地区的1 730份禽源样品,进行增菌、纯化、PCR鉴定、血清学试验及生化试验,并对7株血清型有变异的疑似鸡白痢沙门菌进行进一步序列测定,对血清型进行分析.结果表明,本次调查中共分离沙门菌87株,包括鼠伤寒沙门菌、德尔卑沙门菌、圣保罗沙门菌、鸡白痢沙门菌及阿巴特图巴沙门菌,并且在鸡白痢沙门菌血清型H相存在Hd、Hg阳性的现象.结果提示,中国禽类沙门菌感染以鸡白痢沙门菌感染为主,且存在血清型变异情况,变异概率呈上升趋势.
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