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首页> 中文期刊> 《畜牧兽医学报》 >肉鸡胫骨软骨发育不良早期钙黏蛋白1(CDH1)差异表达研究

肉鸡胫骨软骨发育不良早期钙黏蛋白1(CDH1)差异表达研究

         
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The aim of this study was to explore the differential expression of cadherin 1 (CDH1) in the growth plate of broiler chickens with tibial dyschondroplasia CTD) induced by thiram at the early stage. Chickens were dissected and growth plates were fixed in 4% paraform respectively in 4 ℃ at days 1, 2 and 6. RNA was extracted from the growth plates of control and thiram-fed chickens. Then differential expression of CDH1 was identified by real-time PCR and immunohis-tology. The results showed that expression of CDHl was up-regulated on the growth plate of thi-ram-diet fed group. CDHl protein synthesis mainly lied in cytoplasm of the prehypertrophic and hypertrophic chondrocyte in control or TD growth plates. No expression was discovered at prolif- erative zone and low expression was detected at calcification zone. Remarkably increased CDH1 was detected in TD growth plates at days 1, 2 and 6 after feeding thiram diet, which mirrored with their mRNA differential expression, and highly increased CDH1 staining was clearly seen at days 2 and 6 respectively. It was suggested that CDH1 was associated with cell adhesion, blood vessel invasion and it also regulated Wnt/p-cat signal transmission with other regulator among en-dochondral bone formation.%为研究福美双诱发肉鸡胫骨软骨发育不良(Tibial Dyschondroplasia,TD)早期钙黏蛋白1(CDH1)的差异表达,基础日粮中添加福美双,在试验第1、2、6天,对试验鸡进行剖杀,迅速采取胫骨生长板故入4％多聚甲醛溶液于4℃固定,做CDH1免疫组化分析；提取对照组和饲喂福美双组的生长板总RNA,采用Real-time PCR对CDH1 基因进行差异表达验证.结果钙黏蛋白1 (CDH1/E-cadherin)基因在TD生长板表达上调,在对照和TD软骨生长板,其蛋白合成主要在前肥大、肥大区软骨细胞质,增殖区软骨细胞无表达,钙化区软骨细胞表达少,在饲喂福美双第1、2、6天其表达增加与定量PCR变化结果相一致,且在饲喂福美双第2、6天呈明显高表达.结果表明CDH1 参与细胞黏附、血管入侵及调节Wnt/β-cat的信号传递,和其它分子共同调节软骨内骨化.

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