本研究旨在检测内源性绵羊肺腺瘤反转录病毒(enJSRV)与干扰紊-τ(IFN-τ)在妊娠早期蒙古绵羊子宫内膜组织的表达以及enJSRV的表达与外周血孕酮水平变化的关系.运用TaqMan实时荧光定量PCR技术和电化学发光法对enJSRV和IFN-τ在妊娠早期蒙古绵羊子宫内膜组织相对表达及孕酮水平进行了测定.实时荧光定量PCR结果显示,enJSRV和IFN-τmRNA在妊娠早期绵羊子宫内膜组织有不同程度的表达.SAS统计学软件分析得出,子宫内膜组织中enJSRV mRNA在妊娠12～14 d(交配日为0 d)表达较高,2～10、16～30 d的表达均低于前者.1FN-τ mRNA仅在妊娠12～25 d表达,14d达其峰值,30 d就不能检出,且差异都极显著(P＜0.01).电化学发光法结果显示,孕酮水平在妊娠2d为0.4 ng· mL-1,以后升高,妊娠8～16 d维持在8.3 ng.mL-1左右,在妊娠19～30 d孕酮水平有所下降,30 d为2.5 ng·mL-1.以上结果提示,子宫内膜组织中enJSRV mRNA的表达与外周血孕酮含量及IFN-τ的变化高度相关,且enJSRV在胎盘的形态发生及生殖生物学方面发挥重要作用.%In order to detect the expression of enJSRV and IFN-T in endometrial tissue of Mongolian sheep as well as relationship of the expression of enJSRV and the variety of progesterone content in peripheral blood during early pregnancy. TaqMan Real-time Quantify PCR was performed on the relative expression of enJSRV and IFN-T in uterine endometrium, as well as progesterone content in peripheral blood was detected by electrochemiluminescence analyses. The results demonstrated that enJSRV and IFN-r mRNA were expressed specifically in uterine endometrium of Mongolian sheep. The mRNA expression level of enJSRV was high on Day 12-14, while lower than the former on Day 2-10 and 16-30 during pregnancy. IFN-T mRNA was expressed on Day 12-25 of gestation with the maximum value on Day 14, and IFN-T cannot be detected on Day 30. Electrochemiluminescence results showed the progesterone level was 0. 4 ng · mL-1 on Day 2 and rose afterwards on Day 8 of gestation, then kept at about 8. 3 ng · mL-1 on Day 8-16 and decreased during Day 19-30 with the value 2. 5 ng · mL-1 on Day 30. These results indicated that the expression of enJSRV mRNA in endometrium was highly correlated with changes in peripheral blood progesterone content and IFN-T. Furthermore, enJSRV plays a significant role during placental morphogenesis and reproductive biology.
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