This study was conducted to the effects and mechanisms of Gaoqin oral liquid(GOL) on LPS-induced fever in minipigs. Forty minipigs were randomly allotted into four groups: Control group, Model group, Dipyrone group and GOL group. There were six minipigs in each group. Fever model in minipigs was reproduced by peritoneal injection of LPS 80 μg · kg-1 twice and there was 12-hour interval. 6 h after LPS was injected, Control group and model group were drenched with saline, intravenous injection (IM) was used to give dipyrone in Dipyrone group, GOL group was drenched with GOL, one time a day and 3 days continuously. The basal body temperature was measured before LPS was injected and then 6 h later, we made the administration, the rectal temperatures were measured at the point of 1, 3, 5, 24, 30, 42 and 54 h after the first administration. Change of clinical symptoms were observed and scored. 54 h after administration, 6 blood samples were collected in each group from precaval vein, TNF-α, IL-1, cAMP and PGE2 in blood serum were measured. The results showed that, compared with model group, clinical symptoms of GOL group were significantly improved; compared with the control group, IL-1, TNF-a and PGE2, cAMP of model group were significantly increased (F<0. 05); com-pared with model group, GOL significantly lowered the levels of TNF-α, cAMP and PGE2 (P< 0. 05), but have no significant effect on IL-1 levels (P<0. 05). The above results suggest that GOL significantly inhibited LPS-induced fever in minipigs. Antipyretic effect may be related to the inhibition of TNF-α in blood serum, and reduce the release of PGE2 and cAMP.%擒要:本研究旨在探索膏芩口服液对内毒素发热小型猪的解热效果及机制.40头试验小型猪,随机分为4组:对照组、模型组、安乃近组、膏芩口服液组,每组10头.采用大肠杆菌内毒素(LPS) 80 μg·kg-1,连续2次,间隔12 h,腹腔注射攻毒复制试验猪发热模型,第1次攻毒6h后,对照组和模型组灌服生理盐水；安乃近组注射安乃近；膏芩口服液组灌服膏芩口服液,1次·d-1,连续3d.分别监测每组6头试验猪的基础体温和攻毒给药后1、3、5、24、30、42、54 h肛温,观察临床症状并评分,并在54 h时,各组试验猪前腔静脉采血,检测血清中TNF-α、IL-1、cAMP、PGE2的含量.结果表明,与模型组相比,膏芩口服液组能显著缓解内毒素所引起的小型猪发热临床症状,降低体温；与对照组相比,模型组中TNF-α、PGE2、cAMP、IL-1显著增加(P＜0.05)；与模型组相比,膏芩口服液组能显著降低血清TNF-α、PGE2、cAMP含量(P＜0.05),但对于IL-1作用不显著(P＞0.05).由此可见,膏芩口服液能明显抑制内毒素所引起的小型猪发热,其解热作用的机制可能与抑制血清细胞因子TNF-α的生成,减少发热介质PGE2和cAMP的释放有关.
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