反相液相色谱(RPLC)是药物分析和分离纯化过程检测的首选方法,选择合适的色谱柱是检测方法的关键.本文综述了C18色谱柱及高交联度聚合物色谱柱选择性研究的一些方法,包括溶剂三角形法,相似系数法,选择性比较法,logK值法和类正相模式法.这些研究方法从本质上研究了色谱柱选择特性的物质基础和影响选择性的因素.高交联度聚苯乙烯色谱柱的研究也可以为分离纯化中常用的吸附树脂选择特性的研究提供借鉴.%Reverse phase liquid chromatography is the first choice in drug analysis and separation process detection,which selectivity of columns is a key factor.This article reviewed some research methods on the selectivity of C18 and hypercrossilinked polystyrene columns including solvent triangle,similarity coefficient,logK and "quasi normal-phase HPLC".These methods have studied the natural of columns selectivity and factors which effect separation.The researches of hypercrosslinked polystyrene columns columns may guide the choice of macroporous adsorbent resin on purify processing.
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