Objective To isolate, purify and determine the structure of inhibitors of VEGFR.Methods We use the ELISA method to find compounds, and Macroporous Resin, RP-PCR to isolate and purify compounds.The structure was determined by spectral analysis.Results Obtained two nanaomycin analogues: nanaomycinαA and nanaomycin βA, both of them have bioactivities.Conclusions NanaomycinαA and nanaomycin βA are known compounds.It is firstly reported about its antagonistic activity of VEGFR.%目的 从链霉菌2754中提取分离和纯化小分子VEGF受体抑制剂,并进行结构鉴定.方法 以酪氨酸酶活性测定方法(EuSA法)为活性跟踪方法,利用大孔吸附树脂反相HPLC等方法进行分离纯化.利用光谱分析,进行结构解析.结果 分离纯化到两个七尾霉素类化合物nanaomycin αA和nanaomycin βA并具有相关活性.结论 Nanaomycin αA和nanaomycin βA为己知化合物,首次报道该化合物具VEGFR拮抗活性.
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