Objective:To copy and evaluate the spleen deficiency syndrome type in obese mice.Method:Replicating obese mice with high-fat diet,and on this basis,according to the spleen deficient fatigue,less food,stay,loose stools and secondary screening eligible obese mice,and through the ELISA method to detect serum total cholesterol,triglycerides,high-density lipoprotein and low density lipoprotein content,observe serum inflammatory factors (interleukin 6,interleukin 10 and tumor necrosis factor alpha) and CD4 +,CD8 +,CD11c + content changes of the spleen,thymus and intestinal corpuscle.Results:The model of obesity mice was 62.96％,23.53％ of which was spleen deficiency syndrome type.The IL-1O,the TNF alpha of these mice is significantly higher than normal group,interleukin-6 was significantly lower than normal group.The CD4 +,CD8 + and CD11c + of their gut peyer's patch were also significantly reduced.Conclusion:This method could better screen spleen deficiency syndrome by obesity animal medel.%目的:建立脾胃虚弱型肥胖小鼠模型并进行评价.方法:复制高脂饮食诱导肥胖模型,在肥胖模型的基础上按照神疲乏力、食少纳呆、便溏等主要脾虚指标进行二次筛选符合条件的肥胖鼠.通过ELISA法检测血清总胆固醇、甘油三酯、高密度脂蛋白及低密度脂蛋白含量,测定血清中炎症因子(白介素-6、白介素10及肿瘤坏死因子-α)及脾脏、胸腺和肠道派氏小体中CD4+、CD8+及CD11c+等含量变化.结果:肥胖鼠成模率62.96％,其中脾胃虚弱型肥胖鼠23.53％.脾虚型肥胖鼠IL-10、TNF-α显著高于正常组,白介素-6显著低于正常组,其肠遭派氏小体中的CD4+、CD8+及CD11c+明显降低.结论:此方法可以较好地筛选出脾胃虚弱型肥胖鼠,为健脾益气治疗肥胖奠定基础.
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