Objective: To observe the effect of Hua-Yu-Xie-Zhuo-Recipe on integrin linked kinase( ILK) in rats with tubulointerstitial fibrosis. Methods; The model of renal obstruction was established by unilateral ureteral obstruction ( UUO) in rats and then divided into five groups, including sham-operation group, UUOZY group[ treated with treated with Chinese medicine of huayuxiezhuo 5. 7g/( kg·d ] , UUOA group[ treated with ferulaic acid piperazidine dispersible tablet 6.7 mg/(kg-d )] ,UUOV group [ treated with Valsartan S.Smg/Ckg·d) ] and. UUO group, The protein and mRNA of ILK in kidney tissue were observed. Results; The expression of ILK of UUO and the treatment groups was increased significantly compared with sham group (P <0. 05) ; the expression of ILK of all treated groups declined notablely compared with model group (P < 0. 05). Hua-Yu-Xie-Zhuo-Recipe group was superior to piperazine ferulate dispersible tablets group ( P < 0.05). There was no markedly difference among other groups ( P > 0. 05 ). Conclusion; Hua-Yu-Xie-Zhuo-Recipe can inhibit the expression of ILK and ILK-mRNA in renal tissue, resulting reduction of ECM. So, Hua-Yu-Xie-Zhuo-Recipe embody its therapeutic effect on renal interstitial fibrosis.%目的:观察化瘀泄浊方对肾间质纤维化实验大鼠整合素连接激酶(ILK)的影响.方法:选用健康、雌性SD大鼠50只,用单侧输尿管结扎方法复制大鼠肾间质纤维化模型,将其随机分为假手术组、化瘀泄浊方组[化瘀泄浊方5.7g/(kg'd)经口灌服]、阿魏酸哌嗪分散片组[给予阿魏酸哌嗪分散片6.7mg/(kg.d)]、缬沙坦组[给予缬沙坦5.3mg/(kg'd)经口灌服]及模型组[给予同等量生理盐水经口灌服],每组10只大鼠.全部大鼠于实验第21天全部杀检,免疫组化及RT-PCR检测肾组织ILK蛋白及mRNA的表达.结果:免疫组化及RT-PCR结果显示,与假手术组比较,模型组和各治疗组ILK的表达显著增强,差异有统计学意义(P<0.05);与模型组比较,化瘀泄浊方组、阿魏酸哌嗪分散片组、缬沙坦组ILK的表达减弱,差异有统计学意义(P<0.05),其中化瘀泄浊方组ILK的表达优于阿魏酸哌嗪分散片组,差异有统计学意义(P<0.05),其他各组间均无明显差异(P>0.05).结论:化瘀泄浊方能通过抑制肾组织ILK蛋白及其mRNA的表达,使病变肾组织细胞外基质合成减少,发挥对肾问质纤维化的治疗作用.
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