草菇双额岩小粪蝇Bifronsina bifrons是当前草菇生产中的重要害虫。本文测定了5个线虫品系在（30±0.5）℃下对草菇双额岩小粪蝇的致死率和LT50。结果表明,各线虫品系在浓度4000 IJs mL 1下的LT50范围为2.13～4.71 d,其中夜蛾斯氏线虫Steinernema feltiae SN品系对双额岩小粪蝇的毒力最大,LT50为2.13 d,侵染5 d后的致死率达86.67%。菇房施用4×106IJs.m 2S.feltiae SN品系和嗜菌异小杆线虫Heterorhabditisbacteriophora H06品系悬浮液于培养基表面6 d后,对双额岩小粪蝇的防治效果分别达到70.37%和45.68%,SN线虫对双额岩小粪蝇的防效与阿维菌素相当。%Mushroom fly,Bifronsina bifrons,is an important insect pest in straw mushroom production in South China.Virulence of five strains of Steinernema and Heterorhabditis to the fly was bioassayed at（30±0.5） ℃ in laboratory.Results showed that LT50values of the five strains to the mushroom fly ranged from 2.13 d to 4.71 d.S.feltiae SN strain was a potential biocontrol agent against the mushroom fly with LT50value of 2.13 d and 86.67% of lethality at the concentration of 4000 IJs mL 1at 5 d after treatment.Field trails demonstrated that when nematode suspensions containing 4×106IJs m 2 were sprayed onto the mushroom beds the day before inoculation,control efficacy of S.feltiae SN strain and H.bacteriophora H06 strain against the mushroom fly at 6 d after treatment was 70.37% and 45.68%,respectively,and the control efficacy of S.feltiae SN strain was almost the same as that of avermectins.
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