The present work aims to investigate the effects of different medium components on rhamnolipid production by anthrone colorimetry quantitative analysis with Pseudomonas aeruginosa strain XJ601,which was isolated from crude oil⁃contaminated sample. The results showed that water⁃soluble and water⁃insoluble carbon sources had been utilized forwere used to productione of rhamnolipids, while carbon sources such as vegetable oils were especially effective to produce rhamnolipid. With rapeseed oil as corbon source,nitrate was better than ammonium chloride as nitrogen source to enhance the production of rhamnolipids.Not only the type of carbon and nitrogen source but also the C/N ratio strongly influenced total rhamnolipid productivity,the highest final rhamnolipid concentration was observed at a C/N ratio of 20.The addition of P was also effective on the biosynthesis of rhamnolipids.The product of rhamnolipids by shake flask culture had good stability at different temperature and pH and different concentration of NaCl, which suggested that rhamnolipids produced by this strain hold much promise for oil recovery operations as well as for oil spill bioremediation.%以1株从原油污染样品中分离获得的铜绿假单胞菌XJ601为研究对象，采用蒽酮比色法定量分析鼠李糖脂，优化其产鼠李糖脂的培养基组成。研究表明：疏水性底物优于亲水性底物，具有更高的鼠李糖脂产量，尤以菜籽油最佳；氮源中，硝酸盐、NH4 Cl能促进鼠李糖脂的合成，以菜籽油为碳源时，最佳氮源为NaNO3；C／N比值在20时，鼠李糖脂产量最高；P元素的微量添加会影响鼠李糖脂的合成。摇瓶培养获得的鼠李糖脂对不同温度、pH及NaCl浓度都具有较好的稳定性，表明其在三次采油及原油污染生物治理等领域具有较好的应用前景。
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