This review summarized the principles, characteristics, progress and the research direction of protein and metabolic pathway. Successful samples in high performance industrial biocatalyst were presented.%综合概括了各种蛋白质改造与微生物代谢途径改造的非理性定向进化技术的原理、特点,介绍了这些技术在高性能工业生物催化剂改造中的应用.
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