Objective The present study examined the genetic contribution of the human β2 bradykinin receptor gene to essential hypertension and identified the association of a -58T/C polymorphism with essential hypertension in a Chinese Han population. Methods The study consisted of 106 hypertensive subjects and 98 age-and sex-matched controls. The distribution of -58T/C polymorphism was measured in patients and controls by using PCR, SSCP, cloning and sequencing. Results The allelic frequencies were 0.58 for the C allele in 0.42 for the T allele in hypertensive subjects, and 0.46 for the C allele and 0.54 for the T allele in normotensive subjects. The allelic frequencies were in Hardy-Weinberg equilibrium. Significant differences in the genotype distribution (P=0.032) and allelic frequencies (P=0.018) between hypertensive and normotensive subjects were seen. Conclusion These results suggest that -58C allele of the human β2 bradykinin receptor gene may be an independent risk factor for essential hypertension in the Chinese Han population.%目的探讨缓激肽β2受体启动子基因-58T/C多态性与中国汉族人群的高血压病相关性。方法运用多酶链式反应、启动子区单链构象多态性和基因克隆、测序等方法了解106例高血压病患者和性别、年龄相匹配的98名健康人中-58T/C基因分布情况。结果等位基因C、T在高血压病患者和健康人的分布频率分别为0.58、0.42和0.46、0.54,基因分布频率符合Hardy-Weinberg平衡。两组人群的基因型和等位基因分布频率存在明显统计学差异(P=0.032、P＝0.018)。结论人类缓激肽β2受体启动子基因-58T/C突变可能是中国汉族人群发生高血压病的独立危险因素。
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