目的 探讨Uba-2在肝癌组织中的差异表达以及过表达和低表达时其对肝癌细胞增殖和侵袭能力的影响.方法 qRT-PCR法检测肝癌组织中Uba-2 mRNA的表达;将Uba-2的过表达载体和siRNA表达载体转染至肝癌细胞HepG2后,MTS实验检测Uba-2对肝癌细胞增殖能力的影响;细胞迁移/侵袭实验检测其对肝癌细胞侵袭转移能力的影响;Western blot法检测肝癌细胞HepG2中TGF-β1～3及VEGF、Snail蛋白的表达.结果 Uba-2在肝癌组织中呈高表达,与癌旁肝组织中的表达差异有显著性(P＜0.01),qRT-PCR法检测Uba-2在HepG2细胞中过表达或低表达;细胞增殖实验数据显示Uba-2表达水平与肝癌细胞HepG2的增殖能力相关,siRNA干扰沉默HepG2细胞中Uba-2的表达会抑制其增殖生长,细胞迁移/侵袭实验数据显示Uba-2过表达能促进肝癌细胞HepG2的侵袭、转移能力,且与阴性对照组细胞相比差异有统计学意义;Western blot结果显示Uba-2的异常表达影响TGF-β2及VEGF、Snail蛋白表达水平.结论 Uba-2基因在肝癌组织中的高表达可能具有促进肝癌细胞增殖及侵袭转移的作用.%Purpose To investigate the effect of differential expression,over expression and low expression of Uba-2 in hepatic carcinoma on the proliferation and invasion of hepatoma cells.Methods The expression of Uba-2 mRNA in clinical specimens was detected by qRT-PCR.Mter transfection of Uba2 over expression vector and siRNA expression vector into hepatoma cell HepG2,the effect of Uba-2 on proliferation of hepatoma cells was detected by MTS assay while the effect on the invasion and metastasis of hepatoma cells was detected by cell migration/invasion assay.The expression levels of TGF-β1-3,VEGF and Snail proteins in hepatoma cell HepG2 were detected by Western blot.Results The expression of Uba-2 was high in hepatic carcinoma.Compared with that in adjacent liver tissues,there were significant differences (P ＜ 0.01).qRT-PCR detected over expression or low expression of Uba-2 in HepG2 cells.Cell proliferation data showed that the expression level of Uba-2 was related to the proliferation ability of hepatoma cell HepG2,and siRNA interferring the expression of Uba-2 in silent HepG2 cells could inhibit its growth.The data of cell migration/invasion showed that over expression of Uba-2 could promote the invasion and metastasis of hepatoma cell HepG2.Compared with cells in the control group,there were statistically significant differences.The results of Western blot indicated that the abnormal expression of Uba-2 affected the expression levels of TGF-β2,VEGF and Snail proteins.Conclusion The high expression of Uba-2 gene in hepatic carcinoma may promote the proliferation,invasion and metastasis of hepatoma cells.
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