目的 检测MMP-7在大肠管状腺瘤和大肠管状腺瘤癌变组织中的表达,探讨其在癌变过程中的作用,为临床诊断、治疗及预后判断提供新的理论依据.方法 采用免疫组化EliVision两步法研究40例大肠黏膜、82例散发性大肠管状腺瘤、76例大肠管状腺瘤癌变组织中MMP-7的表达.结果 大肠黏膜、散发性大肠管状腺瘤伴上皮不同程度异型增生和大肠管状腺瘤癌变组织中MMP-7阳性率分别为2.5%、32.9%、86.8%.随着病变程度的加深表达逐渐增高,差异具有统计学意义(P0.05).结论 大肠管状腺瘤癌变过程中MMP-7的阳性率逐渐升高,MMP-7可能参与大肠管状腺瘤癌变的发生.%Purpose To investigate the expression of MMP-7 in sporadic colorectal tubular adenoma and colorectal tubular adenoma with cancerous changes and to explore their role in the carcinogenesis of colorectal adenoma, in order to provide a theoretic basis for clinical diagnosis, treatment and predicting diagnosis. Methods The expression of MMP-7 was studied in 40 cases of colorectal muco-sa, 82 cases of sporadic colorectal tubular adenoma with different dysplasia, and 76 cases of colorectal tubular adenoma with cancerous changes using the two-step methods of EliVision plus. Results The positive expression rate of MMP-7 was 2. 5% , 32. 9% and 86. 8% respectively in colorectal mucosa, sporadic colorectal tubular adenoma with different dysplasia and colorectal tubular adenoma with cancerous changes, which was all significantly higher in the cases of colorectal tubular adenoma with different dysplasia or with cancerous changes than that in colorectal mucosa ( P 0. 05 ). Conclusions Expression of MMP-7 increases gradually in the carcinogenesis process of sporadic colorectal tubular adenoma, which suggests that MMP-7 may be involved in the malignant transformation.
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