目的:探讨NGX6、ILK和c-Jun在散发性大肠管状腺瘤癌变过程中的可能作用.方法:采用免疫组化法检测22例异型增生性畸变隐窝灶(ACF)、68例大肠管状腺瘤伴上皮不同程度异型增生、21例大肠管状腺瘤癌变和21例正常对照中NGX6、ILK和c-Jun的表达情况.结果:NGX6蛋白在正常对照、异型增生性ACF、大肠管状腺瘤伴上皮异型增生及大肠管状腺瘤癌变组中阳性表达率逐步降低,而ILK蛋白和c-Jun蛋白的阳性表达率则明显升高(P<0.05).异型增生性ACF、大肠管状腺瘤伴上皮轻、中、重度异型增生组中NGX6蛋白的阳性表达率分别为77.27%、70.83%、37.50%、35.00%,其中,大肠管状腺瘤伴上皮中、重度异型增生组中NGX6蛋白的阳性表达率明显低于异型增生性ACF及腺瘤伴上皮轻度异型增生组(P<0.05).异型增生性ACF、大肠管状腺瘤伴上皮轻、中度异型增生组中ILK蛋白的阳性表达率明显低于腺瘤伴上皮重度异型组(P<0.05);c-Jun蛋白阳性表达率在大肠管状腺瘤伴上皮轻、中、重度异型增生和大肠管状腺瘤癌变组明显高于异型增生性ACF(P<0.05).在大肠管状腺瘤癌变过程中NGX6和ILK间呈负相关(r=-0.455,P<0.05);NGX6和c-Jun间呈负相关(r=-0.417,P<0.05);ILK和c-Jun之间呈正相关(r=0.390,P<0.05).结论:NGX6低表达可能与ILK及c-Jun高表达有关,这可能在散发性大肠管状腺瘤形成及癌变过程中发挥一定作用.%Objective: To explore the possible roles of NGX6, ILK, and c-Jun in the carcinogenesis of sporadic colorectal tubular adenoma.Methods: The expression levels of NGX6, ILK, and c-Jun were studied in 22 cases of dysplastic aberrant crypt foci ( ACF ), 68 cases of sporadic colorectal tubular adenoma with dysplasia ( SCTA-D ), 21 cases of sporadic colorectal tubular adenoma with cancerous changes ( SCTA-Ca ), and 21 cases of normal control ( NC ) by the immunohistochemical staining method.Results: The positive expression rate of NGX6 significantly decreased from NC, dysplastic ACF, and SCTA-D to SCTA-Ca.In contrast, the positive expression rates of ILK and c-Jun obviously increased accordingly ( P ＜ 0.05 ).The positive expression rates of NGX6 in dysplastic ACF,mild SCTA-D, moderate SCTA-D, and severe SCTA-D were 77.27％, 70.83％, 37.50％, and 35.00％, respectively.Compared with that of dysplastic ACF or mild SCTA-D, the positive expression rates of NGX6 in moderate and severe SCTA-D significantly decreased ( P ＜ 0.05 ).In severe SCTA-D, ILK expression was also significantly higher than those in dysplastic ACF, mild SCTA-D, and moderate SCTA-D ( P ＜ 0.05 ).The positive expression rates of c-Jun in both SCTA-D and SCTA-Ca were significantly higher as well than that in dysplastic ACF ( P ＜ 0.05 ).In the carcinogenic process of sporadic colorectal tubular adenoma, a negative correlation was found between the expression of NGX6 and ILK ( r = -0.455, P ＜ 0.05 ), as well as between NGX6 and c-Jun ( r = -0.417, P ＜ 0.05 ).On the other hand, a positive correlation was found between the expression levels of ILK and c-Jun ( r = 0.390, P ＜ 0.05 ).Conclusion: The down-regulation of NGX6 as well as the up-regulation of ILK and c-Jun may play important roles in colorectal adenoma formation and carcinogenesis.
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