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首页> 中文期刊> 《临床与实验病理学杂志》 >SCF对高糖环境下豚鼠膀胱Cajal样间质细胞超微结构的影响

SCF对高糖环境下豚鼠膀胱Cajal样间质细胞超微结构的影响

         
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Purpose To investigate the effects of stem cell factor( SCF )on the ultrastructure of interstitial cells of Cajal-Iike cells ( ICCs-Iike ) in the bladder of guinea pig in the high glucose environment. Methods The ICCs-like cells was isolated from the bladder of guinea pig by collagenase digestion, and divided into normal control group( glucose concentration 5 mmol/L )and high glucose group ( glucose concentration 15 mmol/L ). After 72 h culture , the high glucose group was divided into high glucose control group,SCF 50 ng/ ml group and SCF 100 ng/ml group,continued to cultivate for 24 h and 72 h. The ultrastructural changes of ICCs-like cells in groups and different time were ohserved by transmission electron microscope. Results With the increase of culture time.ICCs-like cells in normal control group, no significant changes were observed in the ultrastructure. Compared to the normal control group, the major change of ICCs-like cells in high glucose control group showed as follows : the organelles were decreased; mitochondrion was swelling,inequality m size . vacuoles and even dissolving , endoplasmic reticulum was extended , cytoplasm was dissolving , vacuoles were formed . the scabrosity became shorter or disappeared. SCF groups gradually tended to the normal control group. Conclusion Exogenous SCF may improve the ultrastructural damage of ICCs-like cells in the high glucose environment.%目的 探讨干细胞因子(SCF)对高糖环境下豚鼠膀胱Cajal样间质细胞(ICCs-like)超微结构的影响.方法 胶原酶消化法原代分离培养豚鼠膀胱ICCs样细胞.实验分组:正常对照组(葡萄糖浓度为5 mmol/L)、高糖组(葡萄糖浓度为15 mmol/L),培养基培养72 h后高糖组分为高糖对照组、SCF 50 ng/ml组、SCF 100 ng/ml组,继续培养24 h及72 h.透射电镜观察各组不同时间段ICCs样细胞超微结构.结果 随着培养时间延长,正常对照组ICCs样细胞内超微结构无明显变化;高糖对照组ICCs样细胞内细胞器明显减少,线粒体肿胀、大小不等、空泡样变甚至溶解;内质网扩张,胞质广泛溶解,胞周突起变短乃至消失;而SCF组ICCs样细胞超微结构得到逐步改善,细胞器增多、形态趋于正常对照组,胞周突起增多.结论 外源性SCF干预后对高糖环境造成的ICCs样细胞超微结构破坏有一定的修复或逆转作用.

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