目的 探讨宫颈癌患者T淋巴细胞亚群与自然杀伤细胞(NK细胞)水平和病情的相关性.方法选取226例宫颈癌患者,根据宫颈癌临床分期分为Ⅰ期组(52例)、Ⅱ期组(63例)、Ⅲ期组(57例)及Ⅳ期组(54例)四组.另选同期健康体检者60例女性作为对照组,使用流式细胞仪检测并比较上述各组T淋巴细胞亚群(CD+3、CD+3CD+4、CD+3CD+8、CD+3CD+4/CD+3CD+8)及NK细胞(CD+16CD+56)水平.结果 Ⅱ期组、Ⅲ期组及Ⅳ期组CD+3水平明显低于对照组和Ⅰ期组(P<0.05),且随着分期的增加,CD+3水平逐渐降低(P<0.05);Ⅰ期组、Ⅱ期组、Ⅲ期组及Ⅳ期组CD+3CD+4、CD+3CD+4/CD+3CD+8水平明显低于对照组(P<0.05),且随着分期的增加, CD+3CD+4、CD+3CD+4/CD+3CD+8水平逐渐降低(P<0.05);Ⅱ期组、Ⅲ期组及Ⅳ期组CD+3CD+8水平明显高于对照组与Ⅰ期组(P<0.05),且随着分期的增加,CD+3CD+8水平逐渐升高(P<0.05).Ⅰ期组、Ⅱ期组、Ⅲ期组及Ⅳ期组CD+16CD+56数量明显低于对照组(P<0.05),且随着分期的增加,CD+16CD+56数量逐渐降低(P<0.05).宫颈癌患者临床分期与CD+3、CD+3CD+4、CD+3CD+4/CD+3CD+8及CD+16CD+56水平成负相关(P<0.01),与CD+3CD+8成正相关(P<0.01).结论 T淋巴细胞亚群与NK细胞水平可反映宫颈癌患者的细胞免疫功能,宫颈癌临床分期越晚,细胞免疫功能越低.%Objective To investigate the relationship between T lymphocyte subsets and NK cells and disease condition of patients with cervical cancer.Methods A total of 226 patients with cervical cancer in our hospital were di-vided into stage Ⅰgroup(52 cases),stage Ⅱgroup(63 cases),stage Ⅲgroup(57 cases)and stage Ⅳ group(ca-ses)according to their clinical stage,and 60 cases of healthy women in the same period were selected as control group. The T lymphocyte subsets(CD3 +,CD3 +CD4 +,CD3 +CD8 +,CD3 +CD4 +/CD3 +CD8 +)and NK cells(CD16 +CD56 +)of each group were detected by Flow cytometry and compared.Results The CD3 +level of stage Ⅱ group, stage Ⅲgroup,stage Ⅳgroup were significantly lower than those of control and stage Ⅰgroup(P<0.05),and CD3 +level decreased with the increase of stage(P<0.05).The CD3 +CD4 +,CD3 +CD4+/CD3 +CD8 +levels of stage Ⅰgroup,stageⅡgroup,stageⅢgroup,stageⅣgroup were significantly lower than those of control group(P<0.05),and CD3 +CD4 +,CD3+CD4 +/CD3 +CD8 +levels decreased with the increase of stage(P<0.05).The CD3 +CD8 +levels of stageⅡgroup,stageⅢgroup,stageⅣgroup were significantly higher than those of control and stage Ⅰgroup(P<0.05),and CD3 +level increased with the increase of stage(P<0.05).The CD16 +CD56 +levels of stage Ⅰ group, stage Ⅱgroup,stage Ⅲ group,stage Ⅳ group were significantly lower than those of control group(P<0.05),and CD16 +CD56 +levels decreased with the increase of stage(P<0.05).The clinical stage of cervical cancer was nega-tively correlated with CD3 +,CD3 +CD4 +,CD3 +CD4 +/CD3 +CD56 +and CD56 +CD56 +(P<0.01),and positively correlated with CD3 +CD8 +(P<0.01).Conclusion T lymphocyte subsets and NK cell levels could reflect the cellular immune function of cervical cancer patients,and the clinical stage of cervical cancer is later,the cellular immune func-tion is worse.
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