Objective To observe the effect of Xuebijing on expression of intercellular adhesion molecule-1 (ICAM-1)in the lung tissue of rats with acute lung injury induced by lipopolysaccharide.Methods Forty-five Wistar rats were randomly divided into 3 groups by random number table method:(1)control group(n =15),(2)LPS group (n =15),(3)Xuebijing group(XBJ group)(n =15).Injection of LPS(5 mg/kg)into femoral vein to reproduce ALI model in LPS group and XBJ group.Male Wistar rats of XBJ group were given XBJ 4 mL/kg 30 min before the intrave-nous injection of LPS,whereas control group and LPS group were given equivalent volume normal saline.Five rats in each group were killed at 6 h,12 h and 24 h,which were divided into subgroup I,Ⅱ,Ⅲ.The pathomorphism changes of lung tissue were observed by light microscope,and expression of ICAM-1 in pulmonary was examined by immunohisto-chemistry.Results Expression of ICAM-1 were gradually increased with experimental time prolonged and reached the peak at 24h in LPS group after establishing model.ICAM-1 were significantly lower in XBJ group than LPS group at each time points (P <0.05).Compared to LPS group,the pathological change was significantly reduce in XBJ group under microscope.Conclusion Xuebijing can inhibit the expressions of ICAM-1 and reduce acute lung injury induced by li-popolysaccharide in the lung tissue of rats.%目的：观察血必净对脂多糖诱导的急性肺损伤（ALI）大鼠细胞间粘附分子－1（ICAM-1）表达的影响。方法将45只 Wistar 大鼠采用随机数字表分为三组：（1）健康对照（Control）组（15只）、（2）脂多糖（LPS）组（15只）、（3）血必净（XBJ）组（15只）。LPS 组和 XBJ 组采用股静脉注射 LPS（5 mg／kg）制备大鼠 ALI模型。造模前半小时开始给药，XBJ 组给予血必净4 mL／kg；Control 组和 LPS 组则给予等容积0．9％氯化钠注射溶液。在造模后6 h、12 h、24 h 各随机处死大鼠5只，分别作为Ⅰ、Ⅱ、Ⅲ亚组，光镜下观察肺脏组织形态学变化，免疫组织化学法（IHC）检测肺组织中 ICAM-1蛋白表达。结果造模后 LPS 组随着实验时间的延长，肺组织ICAM-1蛋白呈逐渐增加趋势，至24 h 达到高峰。与 LPS 组相比，血必净组各时间点肺组织 ICAM-1蛋白表达明显降低（P ＜O．05）。光镜显示 LPS 组肺组织病理形态学改变明显，而血必净组则明显减轻。结论血必净可通过抑制脂多糖诱导的急性肺损伤大鼠肺组织增强的 ICAM－1表达减轻脂多糖诱导的大鼠肺损伤程度。
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