Objective To observe the expression of Drp1 in the cardial muscle tissue of diabetic mice and nor-mal mice.Methods Nine male db /db mice were randomly divided into 3 groups(db /db 6week group,db /db 12week group and db /db 18week group).Nine male normal mice were randomly divided into another 3 groups(wt 6week group, wt 12week group and wt 18week group).The expression of Drp1 was detected by real-time PCR,Western Blot and im-munohistochemical.Results Compared to the normal group,the expression of Drp1 was decreased obviously in db /db mice group,and was gradually decreased with age.Conclusions The decreased expression of Drp1 maybe is one of the possible mechanisms of diabetic cardiomyopathy.%目的：观察 db ／db 糖尿病小鼠与正常小鼠心肌组织中动力相关蛋白1（Drp1）表达水平的变化。方法9只雄性 db ／db 糖尿病小鼠，随机分为6周组（db ／db 6week），12周组（db ／db 12week），18周组（db ／db 18week）；另设健康组雄性小鼠9只，随机分为6周组（wt 6week），12周组（wt 12week），18周组（wt 18week），每小组3只。分别利用实时定量 PCR，Western Blot，免疫组织化学的方法检测心肌组织中 Drp1的表达和定位。结果db ／db 糖尿病小鼠分组中 Drp1的表达低于健康组，且随年龄的增长表达逐渐降低。结论糖尿病小鼠中Drp1表达进行性的降低，引起线粒体分裂异常从而导致线粒体的生物功能障碍，可能是糖尿病心肌病发生及发展的重要机制之一。
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