Objective To investigate the association between the polymorphisms in CYP17 gene with late-onset male hypogonadism (LOH)and explore the pathogenesis of LOH.Methods The investigation involved 356 men aged 40-79 years,including 36 cases with LOH.130 healthy middle-aged males were randomly selected.The body mass index (BMI),height,waist circumference (WC)and blood pressure were measured and the levels of Triglycer-ide (TG),serum total cholesterol (TC),fasting blood glucose (FBG),serum total testosterone(T),serum estradiol (E2 )were also detected.The polymorphisms of CYP17 gene were examined by PCR.Results The polymorphisms of CYP17 gene were A1/A1,A1/A2 and A2/A2.The mean SBP,BMI,TG,TC and FBG in the healthy males were sig-nificantly lower (P<0.05),whileT and T/E2ratio were markedly higher compared with the LOH males(P<0.01), There was no significant difference in the level of E2 between the two groups(P>0.05 ).There were no significant difference in the levels of serum T and T/E2 ratio of the different CYP17 genotypes in the normal middle-aged males (P>0.05),while significant difference was found in the different CYP17 genotypes in LOH males (P<0.05),and there was no significant difference in the level of E2 in the different CYP17 genotypes between the two groups(P>0.05).Heterozygous variants (A1/A2)of CYP17 were present in less frequency (33.3%)and homozygous (A2/A2)variants were higher frequently (47.3%)found in LOH,and the results were significant(P<0.05).Conclu-sion Polymorphisms in CYP17 gene may be associated with serum T concentrations in LOH cases,and low levels of serum T and T/E2 ratio might possibly act as risk factors for LOH.%目的：探讨细胞色素P450酶17（CYP17）基因多态性与迟发型男性性腺功能减退症（LOH）的关系，研究LOH的发病机制。方法纳入年龄在49～79岁的中老年男性356例，其中36例诊断为迟发性性腺功能减退症患者，随机抽取130例正常健康中老年男性，测量身高、体质量、腰围（WC）、血压，测定三酰甘油（TG）、总胆固醇（TC）、空腹血糖（FBG）、血清总睾酮（T）、雌二醇（E2），并应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测CYP17基因多态位点的片段，产物在2％琼脂糖凝胶上电泳确定出CYP17基因型，比较两组各指标之间的差异。结果 CYP17基因呈多态性：A1/A1、A1/A2和A2/A2。正常健康中老年男性的SBP、BMI、TG、TC 和，FBG较 LOH 患者低（P＜0．05），而 T和 T/E2比值较 LOH 组显著升高（P＜0．01），两组间E2差异无统计学意义（P＞0．05）。正常健康中老年男性组CYP17不同基因型之间T和T/E2比值差异无统计学意义（P＞0．05）；LOH组CYP17不同基因型之间T和T/E2比值差异有统计学意义（P＜0．05），两组不同基因型之间E2差异无统计学意义（P＞0．05）。LOH组较正常健康中老年男性组CYP17基因杂合变异型A1/A2呈现低频率分布（33．3％），而纯合变异型A2/A2呈高频率分布（47．3％），差异有统计学意义（P＜0．05）。结论 CYP17基因多态性可能与LOH患者的T水平有关，低水平的T和T/E2比值可能是LOH发病的危险因素。
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