Objective To explore immunophenotyping specificities in acute leucocythemia via flow cytometry. Methods Flow cytometry was used to have immunophenotyping of acute leucocythemia tested and assist in making the diagnosis leukemia detection. Results (1) Acute lymphocytic leukemia was divided into T cells in series of acute leukemia (T-ALL) and B cell lines acute leukemia (B-ALL) by using specific antibodies. (2) with specific antibodies, acute myelogenous leukemia (AML) can be diagnosed more clear. (3)Acute lymphocytic leukemia (ALL) with myeloid antigen expression ( My-ALL), AML antigen expression from Ly (AML) and mixed leukemia were found. Conclusion Immunophenotype can reflect the heterogeneity of leukemia cells more distinctly and consequently , enhance the exactness of diagnosis.%目的 探讨急性白血病流式细胞术免疫分型的特点及临床意义.方法 100例急性白血病,采用流式细胞术进行急性白血病免疫表型检测.结果 ①用特异性抗体将急性淋巴系白血病分为T细胞系列急性淋巴细胞白血病( T-ALL)和B细胞系急性淋巴细胞白血病(B-ALL).②用特异性抗体使急性髓细胞白血病(AML)的分型更明确.③发现急性淋巴细胞白血病(AL)中伴髓系抗原表达(My-ALL)、AML中伴淋系抗原表达( Ly AML)和混合型白血病.结论 免疫学分型能反映白血病细胞的特征,提高了诊断的准确性.
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