目的:探讨老年Wistar大鼠脂联素与血脂代谢紊乱及脂蛋白脂肪酶(LPL)的相关性.方法:根据月龄,将Wistar大鼠分为老年组(20个月龄)、中年组(7个月龄)和青年组(3个月龄).分别测定各组大鼠血清空腹血糖(FBG)、血脂和脂联素.应用real-time PCR检测大鼠皮下脂肪组织脂联素mRNA、LPL mRNA及肝脏LPL mRNA的表达水平.结果:随着月龄增加,血清脂联素及脂肪脂联素mRNA、LPL mRNA及肝脏LPL mRNA表达水平逐渐下降;老年组上述指标显著低于青年组(P＜0.01).老年组总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、极低密度脂蛋白胆固醇(VLDL-C)、FBG水平高于青年组,高密度脂蛋白胆固醇(HDL-C)低于青年组(P＜0.05).Pearson相关分析显示,血清脂联素与脂肪LPLmRNA、肝脏LPL mRNA和HDL-C正相关(P＜0.05),与体质量、月龄、TC、LDL-C、VLDL-C和TG负相关(P＜0.05);脂肪脂联素mRNA与脂肪LPL mRNA、肝脏LPL mRNA、血清脂联素、HDL-C正相关(P＜0.05),与体质量、月龄、TC、LDL-C、VLDL-C负相关(P＜0.05);脂肪LPL mRNA与脂肪脂联素mRNA、肝脏LPL mRNA、血清脂联素、HDL-C正相关(P＜0.05),与体质量、月龄、TC、LDL-C、VLDL C、TG负相关(P＜0.05).多元逐步回归分析显示,血清脂联素与VLDL-C呈独立负相关(P＜0.05);脂肪脂联素mRNA与TC、月龄独立负相关(P＜0.05);脂肪LPL mRNA与月龄独立负相关(P＜0.05).结论:老年大鼠皮下脂肪和血清脂联素水平降低;脂联素水平下降与老年大鼠脂代谢紊乱有关,其可能通过调节LPL表达来影响血脂代谢.%Objective:To explore the relationship between adiponectin and dyslipidemia,lipoprotein lipase (LPL) in aged Wistar rats.Methods.Wistar rats were divided into the aged group (20 months old),the middle-aged group (7 months old) and the young group (3 months old) according to the age.Serum fasting blood glucose (FBG),blood lipid,and adiponectin were measured in each group.The expression levels of adiponectin mRNA and LPL mRNA in the subcutaneous adipose tissue and LPL mRNA in the liver were detected by real-time PCR.Results:With the increase of age,the expression levels of serum adiponectin and fat adiponectin mRNA,LPL mRNA and liver LPL mRNA gradually decreased;the above indexes in the aged group were significantly lower than those in the young group (P＜0.05).The levels of total cholesterol (TC),triglyceride (TG),low density lipoprotein cholesterol (LDL-C),very low density lipoprotein cholesterol (VLDL-C),and FBG of the aged group were higher than those of the young group (P＜0.05),and the level of high density lipoprotein cholesterol (HDL-C) of the aged group was lower than that of the young group (P＜0.05).Pearson correlation analysis showed that serum adiponectin was positively correlated with fat LPL mRNA,liver LPL mRNA,and HDL-C (P＜0.05),and negatively correlated with body weight,age,TC,LDL-C,VLDL-C,and TG (P＜0.05).Fat adiponectin mRNA was positively correlated with fat LPL mRNA,liver LPL mRNA,serum adiponectin,and HDL-C (P＜0.05),and negatively correlated with body weight,age,TC,LDL-C,and VLDL-C (P＜0.05).Fat LPL mRNA was positively correlated with fat adiponectin mRNA,liver LPL mRNA,serum adiponectin and HDL-C (P＜0.05),and negatively correlated with body weight,age,TC,LDL-C,VLDL-C,and TG (P＜0.05).Multiple stepwise regression analysis showed that there was an independent negative correlation between serum adiponectin and VLDL-C (P＜ 0.05);there was an independent negative correlation between fat adiponectin mRNA,TC and age (P＜0.05);there was an independent negative correlation between fat LPL mRNA and age (P＜0.05).Conclusions:The level of adiponectin in subcutaneous fat and serum decreased in aged rats,and the decrease of adiponectin may be related to dyslipidemia in aged rats.It may affect the blood lipid metabolism by regulating the expression of LPL mRNA.
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