Objective:To study the expression of Dickkopf-l(DKKl) in tissue and scrum samples from patients with intrahc-patic cholangiocarcinoma and its relationship with clinical features. Methods;Kxprcssion of DKKl Mrna in 16 cases of intrahc-patic cholangiocarcinoma was detected by quantitative real-time polymcrasc chain reaction (Qrt-PCR). Scrum levels of DKKl in 15 intrahepatic cholangiocarcinoma patients before operation and 20 healthy volunteers were measured by enzyme-linked im-munosorbent assay (KLISA). Kxprcssion of DKKl was further analyzed by immunohistochemistry in tumor samples from 50 cases of intrahepatic cholangiocarcinoma. Finally* the relationships between DKKl expression and clinical characteristics were analyzed. Results; In 16 cases of intrahepatic cholangiocarcinoma, DKKl Mrna levels were significantly higher in tumor tissues than in pcritumoral tissues (P展开▼
