Objective:To investigate the transforming growth factor β1 (TGF-β1) levels and changes of immune function with cancer stage,histological type of cancer in the non-small cell lung carcinoma patients. Methods: A total of 87 hospitalized patients which were histological confirmed non-small cell lung carcinoma (NSCLC), based on the 2009 International Union A-gainst Cancer (UICC) and the International Lung Cancer Research (IASLC) published the seventh edition of the international staging of lung cancer, staging into the group I -IV of patients; also included 42 cases of healthy control subjects. Enzyme-linked immunosorbent assay (ELISA) in peripheral blood fasting TGF-β1 content. Peripheral blood by flow cytometry in CD3+ , CD4+ , CD8+, CD4+ / CDS+ and CD4+ CD25+ cell percentage, and stratified analysis. Results: NSCLC patients with CD3+ , CD4+, CD8+ cells was significantly lower than the controls (P<0. 01); TGF-β1 levels and CD4+ CD256 cells was higher (P<0. 01); NSCLC patients with stage I and II CD3+ , CD4+ , CDS+ cells was higher than in stageIII and IV (P< 0.01); and TGF-β1 levels and CD4+ CD25+ cells was lower than in stage III and IV (P<0.01); but I and II of room, III and IV was no significant difference between (P> 0. 05). Between the different pathological types of TGF-β1 levels and CD3+ , CD4+, CD8+, CD4+ /CD8+ and CD4+ CD25+ cells was not significantly different (P> 0. 05). Conclusions: NSCLC patients with the TGF-β1 and CD4+ CD25+ levels and the patients immune function is a negative correlation, TNM stage later, the immune function, the lower, TGF-01 levels and CD4+ CD25+ cells are higher; but TGF -β1 levels in NSCLC are no difference between different pathological types.%目的:探讨非小细胞肺癌(NSCLC)患者外周血中转化生长因子β1(TGF-β1)水平与机体免疫功能变化及与NSCLC分期、病理类型的关系.方法:87例病理证实为NSCLC并依据2009年国际抗癌联盟和国际肺癌研究会公布的第7版肺癌国际分期法为Ⅰ～Ⅳ期的患者；42例健康人为对照组.应用酶联免疫吸附(ELISA)法检测空腹外周静脉血中TGF-β1含量.采用流式细胞仪检测外周血中CD3+、CD4+、CD8+、CD4+/CD8+和CD4+ CD25+细胞比例,并进行分层分析.结果:NSCLC患者CD3+、CD4+、CD8+细胞比例显著低于对照组(P＜0.01)；TGF-β1含量和CD4+ CD25+细胞比例显著高于对照组(P＜0.01)；NSCLC Ⅰ期和Ⅱ期患者CD3+、CD4+、CD8+细胞比例显著高于Ⅲ期和Ⅳ期患者(P＜0.01)；而TGF-β1水平和CD4+ CD25+细胞比例显著低于Ⅲ期和Ⅳ期患者(P＜0.01)；但Ⅰ期和Ⅱ期之间、Ⅲ期和Ⅳ期之间差异无统计学意义(P＞0.05).在不同病理类型之间TGF-β1水平和CD3+、CD4+、CD8+、CD4+/CD8+和CD4+ CD25+细胞比例均差异无统计学意义(P＞0.05).结论:NSCLC患者外周血中TGF-31和CD4+ CD25+水平与患者的免疫功能呈负相关,TNM分期越晚,机体细胞免疫功能越低,TGF-β1水平和CD4+ CD25+细胞比例越高；但TGF-β1含量在NSCLC不同病理类型间差异无统计学意义.
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