Objective:To investigate the mRNA expression of the WIF-1 gene and the methylation of its promoter in breast can-cer, and to determine the correlation between the epigenetic aberrant WIF-1 DNA methylation and the clinicopathological significance of WIF-1 in breast cancer . Methods:RT-PCR and sensitive methylation-specific-PCR (MSP) were used to detect WIF-1 mRNA ex-pression and the methylation of the WIF-1 promoter in 30 breast cancer samples as well as in tumor-adjacent tissue samples and 9 be-nign breast tissues. Results:The WIF-1 mRNA expression in 30 breast cancer samples significantly decreased compared with those of the other two groups. In addition, WIF-1 methylation was more frequent in breast-tumor tissues compared with those in tumor-free tis-sues. Meanwhile, WIF-1 mRNA expression in breast cancer tissues involved the abnormal methylation of its promoter. Clinicopatholog-ical correlation analysis showed that the abnormal methylation of the WIF-1 gene promoter was not associated with age, TNM stage, histotype, or lymph node metastasis. Conclusion:WIF-1 mRNA expression loss due to abnormal methylation may be a crucial factor in breast cancer development and can thus be used in the prognosis and progression of the disease.%目的：研究WIF-1（Wnt inhibitory factor-1）mRNA在乳腺癌组织中的表达及其启动子区域甲基化情况，进一步探讨WIF-1基因甲基化与乳腺癌临床病理特征的关系。方法：收集2009年9月1日至2009年12月30日青岛大学附属医院乳腺外科手术切除新鲜组织标本69例，其中良性病变组织9例，乳腺癌及癌旁组织各30例，应用RT-PCR及甲基化特异性PCR（methyla-tion specific PCR，MSP）检测乳腺癌组织、相应癌旁组织和乳腺良性病变组织中WIF-1mRNA表达及其启动子甲基化情况。结果：癌组织中WIF-1基因表达率明显低于相应癌旁组织及乳腺良性病变组织，具有显著性差异（χ2=41.786，P<0.05）；与其他两组相比甲基化率在癌组织中明显升高（矫正χ2=16.484，P<0.05）；WIF-1基因表达下降与其异常甲基化存在明显关联（P=0.023）；WIF-1异常甲基化与乳腺癌发病年龄、肿瘤分级、组织分型和淋巴结转移无相关性（P>0.05）。结论：异常甲基化可能是乳腺癌WIF-1基因表达下降的重要原因，是乳腺癌发生、发展的重要机制。
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