目的 为探讨年龄和性别因素对SJ5-SPF鸡生理生化指标的影响.方法 采用全自动血液分析仪对不同周龄SJ5-SPF鸡血液生化指标进行测定,包括谷丙转氨酶( ALT) 、碱性磷酸酶( ALP) 、谷氨酰转移酶( GGT) 、谷草转氨酶( AST) 、乳酸脱氢酶( LDH) 、总蛋白( TP) 、球蛋白( GLB) 、白蛋白( ALB) 、总胆红素( TB) 、直接胆红素( DB) 、间接胆红素( IBIL) 、葡萄糖( GLU) 、甘油三酯( TG) 、胆固醇( TCH) 、尿素氮( BUN) 、肌酐( CRE) 、钾(K)、钠(Na)、钙(Ca),共19项.同时还用RM6240C多道生理信号采集处理系统对不同周龄SJ5-SPF鸡的体温(T)、呼吸频率(R)、心率(HR)、舒张压(DBP)和收缩压(SBP)进行测定.结果 (1)生理常数所测定体温、呼吸频率、心率、舒张压和收缩压五项指标中,组间相比,4 周龄与20、25、40 周龄比较均有显著性差异( P ＜0. 05),同一周龄雌雄间只有体温没有差异;舒张压只在40周龄存在极显著性差异(P ＜ 0. 01);心率在4周龄、20周龄和25周龄雌、雄间存在极显著性差异(P ＜ 0. 01);而呼吸频率和收缩压雌、雄间在4个周龄均有显著性差异(P ＜ 0. 05). (2)血液生化指标测定19项显示,组间相比,雌性各周龄之间只有谷丙转氨酶差异无显著性(P＞ 0. 05),雄性仅有谷丙转氨酶和葡萄糖差异无显著性(P＞ 0. 05),其余生化指标均存在一定的差异;同一周龄雌雄间相比,谷氨酰转移酶、谷丙转氨酶、谷草转氨酶、总胆红素、直接胆红素、间接胆红素、葡萄糖、尿素氮和钠只有1个周龄差异有显著性(P ＜0. 05),乳酸脱氢酶、总蛋白、球蛋白、白蛋白和肌酐在2 个周龄差异有显著性(P ＜0. 05),碱性磷酸酶、甘油三酯和钙在3个周龄差异有显著性(P ＜0. 05),而胆固醇和钾在4个周龄差异有显著性(P ＜0. 05).结论 本研究测定的结果可为SJ5-SPF鸡的疾病诊断、动物检疫和相关的研究提供科学依据.%Objective To study the effect of age and sex on physiological and biochemical indexes of SJ5-SPF chickens at the age of different weeks. Methods Blood biochemical indexes of SJ5-SPF chickens at the age of different weeks were measured using an automatic blood analyzer, including 19 items: ALT, ALP, GGT, AST, LDH, TP, GLB, ALB, TB, DB, IBIL, GLU, TG, TCH, BUN, CRE, K+, Na+, and Ca2+. At the same time, the body temperature (T), respiratory frequency (R), heart rate (HR), diastolic pressure (DBP) and systolic pressure (SBP) of the SJ5-SPF chickens were measured using a RM6240C multi-channel physiological signal acquisition and processing system. Results (1) Among the physiological parameters, the body temperature, respiratory rate, heart rate, diastolic blood pressure and systolic blood pressure showed significant differences between the 4-week old chickens and the chickens at ages of 20, 25, and 25 weeks (P ＜ 0. 05), but there were significant differences between the males and females at the same weeks of age except body temperature. The diastolic blood pressure was only significantly different in the 40-week old male and female chickens (P ＜ 0. 01). The heart rates showed significant differences between the male and female chickens at 4, 20 and 25 weeks of age (P ＜ 0. 01). The respiratory rate and systolic blood pressure in the 4-week old male and female chickens were significantly different (P ＜ 0. 05). (2) The 19 blood biochemical indexes showed significant differences between the male and female chickens: the GGT, ALT, AST, TB, DB, IBIL, GLU, BUN and Na+at an week-age (P＜ 0. 05), the LDH, TP, GLB, ALB and CRE at two different week-ages (P＜ 0. 05), the ALP, TG and Ca2+at three different week-ages (P＜ 0. 05), and the TCH and K+at four different week-ages (P＜ 0. 05). Conclusions The above results can provide a scientific basis for diagnosis of SJ5-SPF chicken disease, animal quarantine and other related research.
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