目的：探讨miR－34a通过Notch信号通路影响乳腺癌细胞的生物学行为。方法乳腺癌细胞MCF－7分为miR－34a及Control组，分别利用脂质体转染miR－34a mimics及miR－34a NC，RT－PCR法检测细胞中miR－34a表达，MTT、Hoechst 染色及 transwell 法分别检测细胞活力、凋亡及侵袭情况；western blot 检测细胞中 Notch1， Notch2，Dll1及Jagged－1蛋白表达情况。结果与Control组比较，miR－34 mimics组中miR－34a表达量上调，细胞皱缩，变圆变亮，细胞活力降低（P＜0．01），细胞凋亡率显著提高（P＜0．01），细胞侵袭数目减少（P＜0．01），Notch1， Notch2，Dll1及Jagged－1蛋白表达量都显著降低（P＜0．01）。结论 miR－34a mimics通过抑制Notch信号通路进而诱导MCF－7乳腺癌细胞凋亡并抑制其侵袭。%Objective To explore effect of miR-34a on the biological behavior of breast cancer cell MCF-7 by Notch signal pathway .Methods The MCF-7 cells were divided into miR-34a and Control groups .The miR-34a mimics and miR-34a NC were transfected by liposome .The expression of miR-34a in MCF-7 cell was examed by RT-PCR.The cell viability was detected by MTT .The cell apoptosis was examed by Hoechst staining .The cell invasion was detected by transwell method.The expression of Nothch1, Nothc2, Dll1 and Jagged-1 was detected by western blot.Results Compared with Control group, the expression of miR-34a was up-regulated (P<0.01), Cell shrinkage, rounded brighten, the cell viability was decreased ( P<0.01 ) , cell apoptotic rate was increased ( P <0.01 ) , cell invasion number was reduced (P<0.01), the expression of Notch1, Nothc2, Dll1 and Jagged-1 was down-regulated (P<0.01).Conclusion miR-34a mimics induced MCF-7 cell apoptosis and inhibited cell invasion via inhibition of Notch signal pathway .
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