Objective To observe the effect of Xuebijing treat on the multiple organ dysfunction syndrome ( MODS) , and the change of NF-κB mRNA in lung tissue and its significance, therefore to assess the role of Xuebijing in the prevention and treatment of highland MODS ( H-MODS) .Methods After anesthesia, 24 ba-ma pigs were randomly divided into blank control group, H-MODS model group and the Xuebijing group, each group containing 8 pigs.LPS was used to induced H-MODS pig model.Xuebijing group was given: the 3 mL/kg Xuebijing, twice/day for three days; blank control group and model group was given same amont of physiological saline for three days.RT-PCR was used to measure the change of NF-κB mRNA in lung tissue.Results ①The animal mortality in model group and the Xuebijing group was 33.3%and 16.6%, respectively.②Compared with control group, 24 h model group showed alveolar cavities visible diffuse blood stasis, including a lot of red blood cells and a small amount of neutrophil infiltration and many macrophages.24 h model group also showed increased alveolar interval, capillaries expansion congestion, but these pathological changes was lightly in the Xuebijing group.③The model group and the Xuebijing group of white blood cell ( WBC) showed“Scoop” type change.The WBC rise range in model group was obviously higher than that in control group in 48 h.④The NF-κB mRNA expression was obviously higher in the model group and Xuebijing group than that in control group in 24 h (P<0.01);the NF-κB mRNA expression in 24 h rise range in Xuebijing group was statistically significant lower than that in model group (P<0.05);in 72 h model group and the Xuebijing group, the NF-κB mRNA expression was still higher than control group, and the most obvious rising was model group ( P<0.05).Conclusion The intervention treatment with Xuebijing in model of LPS-induced H-MODS, not only reduces the mortality rate of H-MODS model ba-ma pigs, reduces inflammation caused by LPS, and the NF-κB mRNA expression in lung tissue, but also improves the survival rates of H-MODS.%目的：观察血必净治疗巴马小香猪高原多器官功能障碍综合征（ H－MODS）模型的疗效及治疗前后肺组织中NF－κB mRNA的表达，进一步揭示血必净对H－MODS的防治作用。方法24只巴马小香猪麻醉后随机抽取8只作为空白对照组（ A组），其余16只采用内毒素（ LPS）制作H－MODS模型后，随机分为模型组（ B组）、血必净治疗组（ C组），每组8只。血必净治疗组给予血必净注射液3 mL／kg，2次／d；对照组及模型组给予等体积生理盐水，2次／d；共治疗3 d。于不同时间段观察巴马小香猪血白细胞的变化趋势。采用RT－PCR法测定肺组织NF－κB mRNA表达量变化。结果①模型组、血必净治疗组动物死亡率分别为33．3％、16．6％。②与对照组比较，24 h模型组肺泡腔内可见弥散性瘀血，巨噬细胞增多，且有大量红细胞渗出及少量中性粒细胞浸润，肺泡间隔增宽，毛细血管扩张充血，24 h血必净治疗组肺泡腔内出血较少，肺泡壁模糊，有少量红细胞渗出，肺泡间隔仍有增厚。③模型组及血必净治疗组血白细胞（ WBC）呈“勺型”变化。模型组48 h WBC变化幅度较治疗组更明显（ P＜0．05）。④与对照组比较，模型组、治疗组24 h NF－κB mRNA表达量均显著上升（P＜0．01）；与模型组比较，治疗组24 h NF－κB mRNA表达量升高幅度较低（ P＜0．05）；模型组、治疗组72 h NF－κB mRNA表达量仍高于对照组，以模型组升高最明显（ P＜0．05）。结论血必净可明显减轻内毒素所诱导的H－MODS模型动物死亡率，减轻内毒素所致的炎症反应，下调肺组织NF－κB mRNA的表达量，提高H－MODS的救治成功率。
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