Objective To observe the change in IL-1β,IL-13mRNA expression in drowning rat lungs and serum,so as to investigate the significance of IL-1β and IL-13 mechanism in the development of drowning.Methods SD rats were randomly divided into control group,drowning group.Then using TaqMan probe method to determine the expression of IL-1β and IL-13 mRNA in Right lower lobe of lung tissue and the serum of right ventricle,which were extracted respectively from each group of rats.Results (1) The lung tissue morphological changes:Typical appearance signs and anatomy of drowning group meet ante-mortem drowning feature.(2) The expression of IL-1β,IL-13 in lung tissue:compared with the control group,the expression of IL-1β and IL-13 were slightly decreased,which has no statistical significance.(3) The expression of IL-1β and IL-13 in serum:compared with the control group,the expression of IL-1β and IL-13 were significant increased,both of which has statistical significance.Conclusion (1)The expression of IL-1β and IL-13 were decreased in lung tissue may be due to drowned rats present compensatory anti-inflammatory response syndrome which causes immune incompetent performance.(2) The expression of IL-1β and IL-13 were significant increased in serum may be relate to drown stress and drowning associated acute lung injury after traumatic stress.%目的 检测大鼠肺组织和血清中IL-1β、1L-13mRNA表达变化,探讨IL-1β、IL-13mRNA的变化情况与溺死的关系.方法 12只SD大鼠随机分为对照组、溺死组(实验组).分别提取各组大鼠右侧肺下叶及右心室血清,应用TaqMan探针法检测肺组织及右心室血清IL-1 β、IL-13mRNA的表达情况.结果 (1)溺死组大鼠肉眼观察:体表征象及解剖所见符合生前溺死特征.(2)肺组织中IL-1β、IL-13mRNA表达检测:与对照组比较,实验组IL-1β、IL-13表达呈下降趋势,差异无统计学意义(p＞0.05).(3)血清中IL-1β、IL-13mRNA表达检测:与对照组比较,实验组IL-1β表达显著升高(p＜0.01)、IL-13表达显著升高(p＜0.01).结论 (1) IL-1β、IL-13mRNA在溺死组肺组织中表达呈下降趋势,可能是大鼠溺死出现代偿性抗炎反应综合症导致免疫无能的表现.(2) IL-lβ、IL-13mRNA在血清中表达明显升高,可能与溺水应激及溺死后急性肺损伤创伤应激有关.
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