目的 建立小体积液相萃取-GC/MS法检验尿液中的MDPV分析方法.方法 将添加MDPV标准品的尿液调pH值为9,采用小体积二氯甲烷进行提取,有机相直接进行GC/MS分析.结果 MDPV的线性范围为0.05μg/mL-0.20μg/mL(R2=0.9965),检出限为0.02μg/mL,提取回收率高于85%,精密度小于10%.结论 本文建立的方法操作简单、灵敏、快速、经济,适用于吸毒检验工作中尿液中MDPV的检验.%Objective The method for the analysis of Methylenedioxypyrovalerone (MDPV) in urine by liquid-phase small-extraction- GC/MS have been developed and studied . Methods A 1-mL of urine sample was adjusted to pH9 with sodium bicarbonate, and was extracted with 50μL dichloromethane. The mixture centrifuged at 14000g for 2 min . A 1-μL organic phase was injected into the GC/MS system. Results The calibration curves showed good linearity in range of 0.05μg/mL~0.20μg/mL , and the limit of detection was 0.02μg/mL, and the RSD was 3.86~5.69%, and the recovery rate was 86.5~92.8 %. Conclusion The method is sensitive, accurate and was easy to operate for fast detection of MDPV in human urine.
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