目的：研究勒福特王水消解法对动物器官中硅藻检验的法医学应用。方法将勒福特王水消解-滤膜富集-扫描电镜观察法与传统强酸消解-离心富集-光学显微镜观察法比较，对生前入水和死后入水的实验动物肺、肝和肾组织中的硅藻数量、检验时间、检出率以及观察效果进行研究，并进行统计学分析。结果生前入水组用勒福特王水消解-滤膜富集-扫描电镜观察法检出肺、肝、肾组织中的硅藻数量均多于传统方法（P0.05），观察效果优于传统方法。结论勒福特王水消解-滤膜富集-扫描电镜观察法具有简单、高效、定性定量分析准确的特点，该方法成本低廉，操作简单，具有较好的实用价值。%Objective The forensic application of Lefort aqua regia digestion method detecting diatom in rabbits’s organs. Methods Using the Lefort aqua regia digestion-membrane ifltrition-SEM observation method and the traditional acid digestion-centrifugation-light microscopy observation method to detect diatom of drowning or postmortem immersion rabbits’s organs(lung,liver,kidney), and make statistical analysis of diatom number, consuming time, digestive capability and ifne structure of diatoms. Results The diatom number of Lefort aqua regia digestion method was superior to conventional acid digestion method, with the difference being statistically signiifcant (P0.05), and have a better observation effect. Conclusion The Lefort aqua regia digestion method for diatom extraction was helpful for diatom identiifcation., and useful in drowning test by conventional and economicly.
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